Antigen depot is not required for alum adjuvanticity

Hutchison, S., Benson, R.A., Gibson, V.B., Pollock, A.H., Garside, P. and Brewer, J.M. (2012) Antigen depot is not required for alum adjuvanticity. FASEB Journal, 26(3), pp. 1272-1279. (doi:10.1096/fj.11-184556)

Hutchison, S., Benson, R.A., Gibson, V.B., Pollock, A.H., Garside, P. and Brewer, J.M. (2012) Antigen depot is not required for alum adjuvanticity. FASEB Journal, 26(3), pp. 1272-1279. (doi:10.1096/fj.11-184556)

[img]
Preview
Text
1272.full.pdf

1MB

Publisher's URL: http://dx.doi.org/10.1096/fj.11-184556

Abstract

Alum adjuvants have been in continuous clinical use for more than 80 yr. While the prevailing theory has been that depot formation and the associated slow release of antigen and/or inflammation are responsible for alum enhancement of antigen presentation and subsequent T- and B-cell responses, this has never been formally proven. To examine antigen persistence, we used the chimeric fluorescent protein EαGFP, which allows assessment of antigen presentation in situ, using the Y-Ae antibody. We demonstrate that alum and/or CpG adjuvants induced similar uptake of antigen, and in all cases, GFP signal did not persist beyond 24 h in draining lymph node antigen-presenting cells. Antigen presentation was first detectable on B cells within 6–12 h of antigen administration, followed by conventional dendritic cells (DCs) at 12–24 h, then finally plasmacytoid DCs at 48 h or later. Again, alum and/or CpG adjuvants did not have an effect on the magnitude or sequence of this response; furthermore, they induced similar antigen-specific T-cell activation in vivo. Notably, removal of the injection site and associated alum depot, as early as 2 h after administration, had no appreciable effect on antigen-specific T- and B-cell responses. This study clearly rules out a role for depot formation in alum adjuvant activity.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Brewer, Professor James and Garside, Professor Paul and Gibson, Miss Vivienne and Benson, Dr Robert and Hutchison, Dr Sharon
Authors: Hutchison, S., Benson, R.A., Gibson, V.B., Pollock, A.H., Garside, P., and Brewer, J.M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:FASEB Journal
ISSN:0892-6638
ISSN (Online):1530-6860
Published Online:21 November 2011
Copyright Holders:Copyright © 2012 The Authors
First Published:First published in FASEB Journal 2012 26(3):1272-1279
Publisher Policy:Distributed under the terms of the Creative Commons Attribution Non-Commercial License

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
419721Investigating the contributions of inflammation and antigen depot to adjuvant function in vivoJames BrewerBiotechnology and Biological Sciences Research Council (BBSRC)BB/E00069X/1III -IMMUNOLOGY