Antigen depot is not required for alum adjuvanticity

Hutchison, S. , Benson, R.A. , Gibson, V.B. , Pollock, A.H. , Garside, P. and Brewer, J.M. (2012) Antigen depot is not required for alum adjuvanticity. FASEB Journal, 26(3), pp. 1272-1279. (doi: 10.1096/fj.11-184556) (PMID:22106367) (PMCID:PMC3289510)



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Alum adjuvants have been in continuous clinical use for more than 80 yr. While the prevailing theory has been that depot formation and the associated slow release of antigen and/or inflammation are responsible for alum enhancement of antigen presentation and subsequent T- and B-cell responses, this has never been formally proven. To examine antigen persistence, we used the chimeric fluorescent protein EαGFP, which allows assessment of antigen presentation in situ, using the Y-Ae antibody. We demonstrate that alum and/or CpG adjuvants induced similar uptake of antigen, and in all cases, GFP signal did not persist beyond 24 h in draining lymph node antigen-presenting cells. Antigen presentation was first detectable on B cells within 6–12 h of antigen administration, followed by conventional dendritic cells (DCs) at 12–24 h, then finally plasmacytoid DCs at 48 h or later. Again, alum and/or CpG adjuvants did not have an effect on the magnitude or sequence of this response; furthermore, they induced similar antigen-specific T-cell activation in vivo. Notably, removal of the injection site and associated alum depot, as early as 2 h after administration, had no appreciable effect on antigen-specific T- and B-cell responses. This study clearly rules out a role for depot formation in alum adjuvant activity.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Benson, Dr Robert and Brewer, Professor James and Garside, Professor Paul and Gibson, Miss Vivienne and Hutchison, Dr Sharon
Authors: Hutchison, S., Benson, R.A., Gibson, V.B., Pollock, A.H., Garside, P., and Brewer, J.M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:FASEB Journal
ISSN (Online):1530-6860
Published Online:21 November 2011
Copyright Holders:Copyright © 2012 The Authors
First Published:First published in FASEB Journal 2012 26(3):1272-1279
Publisher Policy:Distributed under the terms of the Creative Commons Attribution Non-Commercial License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
419721Investigating the contributions of inflammation and antigen depot to adjuvant function in vivoJames BrewerBiotechnology and Biological Sciences Research Council (BBSRC)BB/E00069X/1III -IMMUNOLOGY