Quantitative characterization of individual microdroplets using surface-enhanced resonance Raman scattering spectroscopy

Syme, C.D., Martino, C., Yusvana, R., Sirimuthu, N.M.S. and Cooper, J.M. (2012) Quantitative characterization of individual microdroplets using surface-enhanced resonance Raman scattering spectroscopy. Analytical Chemistry, 84(3), pp. 1491-1495. (doi:10.1021/ac202705a)

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Abstract

Surface-enhanced resonance Raman scattering (SERRS) spectroscopy is a highly sensitive optical technique capable of detecting multiple analytes rapidly and simultaneously. There is significant interest in SERRS detection in micro- and nanotechnologies, as it can be used to detect extremely low analyte concentrations in small volumes of fluids, particularly in microfluidic systems. There is also rapidly growing interest in the field of microdroplets, which promises to offer the analyst many potential advantages over existing technologies for both design and control of microfluidic assays. While there have been rapid advances in both fields in recent years, the literature on SERRS-based detection of individual microdroplets remains lacking. In this paper, we demonstrate the ability to quantitatively detect multiple variable analyte concentrations from within individual microdroplets in real time using SERRS spectroscopy. We also demonstrate the use of a programmable pump control algorithm to generate concentration gradients across a chain of droplets.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Narayana Mudalige, Dr Sathkumara and Syme, Dr Christopher and Cooper, Professor Jonathan
Authors: Syme, C.D., Martino, C., Yusvana, R., Sirimuthu, N.M.S., and Cooper, J.M.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Analytical Chemistry
ISSN:0003-2700
Published Online:16 December 2011

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
436881The molecular noseAndrew PittEngineering & Physical Sciences Research Council (EPSRC)EP/E032745/1Institute of Molecular Cell and Systems Biology