The Trypanosoma brucei AIR9-like protein is cytoskeleton-associated and is required for nucleus positioning and accurate cleavage furrow placement

May, S. F., Peacock, L., Almeida Costa, C. I. C., Gibson, W. C., Tetley, L., Robinson, D. R. and Hammarton, T. C. (2012) The Trypanosoma brucei AIR9-like protein is cytoskeleton-associated and is required for nucleus positioning and accurate cleavage furrow placement. Molecular Microbiology, 84(1), pp. 77-92. (doi:10.1111/j.1365-2958.2012.08008.x)

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Publisher's URL: http://dx.doi.org/10.1111/j.1365-2958.2012.08008.x

Abstract

AIR9 is a cytoskeleton-associated protein in Arabidopsis thaliana with roles in cytokinesis and cross wall maturation, and reported homologues in land plants and excavate protists, including trypanosomatids. We show that the Trypanosoma brucei AIR9-like protein, TbAIR9, is also cytoskeleton-associated and colocalises with the subpellicular microtubules. We find it to be expressed in all life cycle stages and show that it is essential for normal proliferation of trypanosomes in vitro. Depletion of TbAIR9 from procyclic trypanosomes resulted in increased cell length due to increased microtubule extension at the cell posterior. Additionally, the nucleus was re-positioned to a location posterior to the kinetoplast, leading to defects in cytokinesis and the generation of aberrant progeny. In contrast, in bloodstream trypanosomes, depletion of TbAIR9 had little effect on nucleus positioning, but resulted in aberrant cleavage furrow placement and the generation of non-equivalent daughter cells following cytokinesis. Our data provide insight into the control of nucleus positioning in this important pathogen and emphasise differences in the cytoskeleton and cell cycle control between two life cycle stages of the T. brucei parasite.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Tetley, Dr Laurence and May, Miss Sophie and Hammarton, Dr Tansy
Authors: May, S. F., Peacock, L., Almeida Costa, C. I. C., Gibson, W. C., Tetley, L., Robinson, D. R., and Hammarton, T. C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Molecular Microbiology
Publisher:Wiley-Blackwell Publishing Ltd.
ISSN:0950-382X
ISSN (Online):1365-2958
Published Online:05 March 2012
Copyright Holders:Copyright © 2012 Blackwell Publishing
First Published:First published in Molecular Microbiology 84(1):77-92
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
493121The NDR kinase pathway in Trypanosoma bruceiTansy HammartonMedical Research Council (MRC)G0900239Infection Immunity and Inflammation Life Sciences
403591Regulation of cytokinesis in trypanosoma bruceiTansy HammartonMedical Research Council (MRC)G120/1001III - PARASITOLOGY