Holographic aberration correction: optimising the stiffness of an optical trap deep in the sample

Dienerowitz, M., Gibson, G. , Bowman, R. and Padgett, M. (2011) Holographic aberration correction: optimising the stiffness of an optical trap deep in the sample. Optics Express, 19(24), pp. 24589-24595. (doi: 10.1364/OE.19.024589)

Abstract

We investigate the effects of 1st order spherical aberration and defocus upon the stiffness of an optical trap tens of μm into the sample. We control both these aberrations with a spatial light modulator. The key to maintain optimum trap stiffness over a range of depths is a specific non-trivial combination of defocus and axial objective position. This optimisation increases the trap stiffness by up to a factor of 3 and allows trapping of 1μm polystyrene beads up to 50μm deep in the sample.<p></p>

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Gibson, Dr Graham and Padgett, Professor Miles and Dienerowitz, Dr Maria
• Authors: Dienerowitz, M., Gibson, G., Bowman, R., and Padgett, M.
• College/School: College of Science and Engineering > School of Physics and Astronomy
• Journal Name: Optics Express
• Publisher: Optical Society of America
• ISSN: 1094-4087
• ISSN (Online): 1094-4087
• Published Online: 01 January 2011
• Copyright Holders: Copyright © 2011 Optical Society of America
• First Published: First published in Optics Express 19(24):24589-24595
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
48257	1	Multi-object, high-throughput, spectro-microscopy: Life Sciences Interface	Miles Padgett	Engineering & Physical Sciences Research Council (EPSRC)	EP/H007636/1	Physics and Astronomy