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Dedifferentiation of rat mammary myoepithelial-like cell lines after passage in vivo or cloning in vitro

Jamieson, S., Dunnington, D.J., Ormerod, E.J., Warburton, M.J., and Rudland, P.S. (1986) Dedifferentiation of rat mammary myoepithelial-like cell lines after passage in vivo or cloning in vitro. Journal of the National Cancer Institute, 76 (2). pp. 247-256. ISSN 0027-8874

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Abstract

Myoepithelial-like cell lines from normal mammary glands of neonatal Ludwig Wistar rats, rat mammary (Rama) 401 and Rama 704E, were injected into fat pads of syngeneic animals or were single-cell cloned in vitro. Rama 401 produced tumors that were predominantly composed of elongated cells, while the subclones of both cell lines yielded multilayered structures of elongated cells when grown on floating 0.3% collagen gels in vitro. Immunocytochemical analysis of histologic sections for markers of myoepithelial cells revealed that anti-actin-myosin and human keratin sera failed to stain the Rama 401 tumor cells or subclones of both cell lines on collagen gels, but both were stained with antilaminin serum. Immunofluorescent analysis of cultures of Rama 401 tumors showed that the resulting elongated cells failed to stain with antikeratin serum, but abundant staining was observed with antilaminin and antivimentin sera, as in the tumors. Ultrastructural analysis of the Rama 401 tumor cells identified intermediate junctions and extracellular basement membrane-like material in the vicinity of plasma membrane-associated pinocytotic vesicles, but neither true desmosomes nor myofilamental bundles were observed. Thus growth of rat mammary myoepithelial-like cells as tumors in syngeneic animals or as subclones in vitro can lead to selective loss of myofilaments and prekeratin-containing intermediate filaments. Similar relatively undifferentiated elongated cells may be responsible for some of the cellular heterogeneity observed in certain carcinogen-induced rat mammary tumors.

Item Type:Article
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Jamieson, Dr Susan
Authors: Jamieson, S., Dunnington, D.J., Ormerod, E.J., Warburton, M.J., and Rudland, P.S.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine > Clinical Specialities
Journal Name:Journal of the National Cancer Institute
Journal Abbr.:J. Natl. Cancer Inst.
ISSN:0027-8874
ISSN (Online):1460-2105
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