The role of microtopography in cellular mechanotransduction

McNamara, L.E., Burchmore, R.J.S., Riehle, M.O. , Herzyk, P., Biggs, M.J.P., Wilkinson, C.D.W., Curtis, A.S.G. and Dalby, M.J. (2012) The role of microtopography in cellular mechanotransduction. Biomaterials, 33(10), pp. 2835-2847. (doi:10.1016/j.biomaterials.2011.11.047)

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Abstract

Mechanotransduction is crucial for cellular processes including cell survival, growth and differentiation. Topographically patterned surfaces offer an invaluable non-invasive means of investigating the cell response to such cues, and greater understanding of mechanotransduction at the cell-material interface has the potential to advance development of tailored topographical substrates and new generation implantable devices. This study focuses on the effects of topographical modulation of cell morphology on chromosomal positioning and gene regulation, using a microgrooved substrate as a non-invasive mechanostimulus. Intra-nuclear reorganisation of the nuclear lamina was noted, and the lamina was required for chromosomal repositioning. It appears that larger chromosomes could be predisposed to such repositioning. Microarrays and a high sensitivity proteomic approach (saturation DiGE) were utilised to identify transcripts and proteins that were subject to mechanoregulated changes in abundance, including mediators of chromatin remodelling and DNA synthesis linked to the changes in nucleolar morphology and the nucleoskeleton.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Riehle, Dr Mathis and Burchmore, Dr Richard and McNamara, Dr Laura and Curtis, Professor Adam and Wilkinson, Professor Christopher and Dalby, Professor Matthew and Herzyk, Dr Pawel
Authors: McNamara, L.E., Burchmore, R.J.S., Riehle, M.O., Herzyk, P., Biggs, M.J.P., Wilkinson, C.D.W., Curtis, A.S.G., and Dalby, M.J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Medical Veterinary and Life Sciences > School of Life Sciences
College of Science and Engineering > School of Engineering
Journal Name:Biomaterials
Publisher:Elsevier
ISSN:0142-9612
ISSN (Online):1878-5905
Published Online:16 January 2012
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