Human DDX3 functions in translation and interacts with the translation initiation factor eIF3

Lee, C.S., Dais, A.P., Jedrychowski, M., Patel, A.H. , Hsu, J.L. and Reed, R. (2008) Human DDX3 functions in translation and interacts with the translation initiation factor eIF3. Nucleic Acids Research, 36(14), pp. 4708-4718. (doi:10.1093/nar/gkn454) (PMID:18628297) (PMCID:PMC2504307)

Full text not currently available from Enlighten.

Abstract

The conserved RNA helicase DDX3 is of major medical importance due to its involvement in numerous cancers, human hepatitis C virus (HCV) and HIV. Although DDX3 has been reported to have a wide variety of cellular functions, its precise role remains obscure. Here, we raised a new antibody to DDX3 and used it to show that DDX3 is evenly distributed throughout the cytoplasm at steady state. Consistent with this observation, HA-tagged DDX3 also localizes to the cytoplasm. RNAi of DDX3 in both human and Drosophila cells shows that DDX3 is required for cell viability. Moreover, using RNAi, we show that DDX3 is required for expression of protein from reporter constructs. In contrast, we did not detect a role for DDX3 in nuclear steps in gene expression. Further insight into the function of DDX3 came from the observation that its major interaction partner is the multi-component translation initiation factor eIF3. We conclude that a primary function for DDX3 is in protein translation, via an interaction with eIF3

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Patel, Professor Arvind
Authors: Lee, C.S., Dais, A.P., Jedrychowski, M., Patel, A.H., Hsu, J.L., and Reed, R.
Subjects:Q Science > QP Physiology
College/School:College of Medical Veterinary and Life Sciences
Journal Name:Nucleic Acids Research
ISSN:0305-1048
ISSN (Online):1362-4962
Published Online:15 July 2008

University Staff: Request a correction | Enlighten Editors: Update this record