Differential in-gel electrophoresis (DIGE) analysis of human bone marrow osteoprogenitor cell contact guidance

Kantawong, F., Burchmore, R.J.S. , Wilkinson, C.D.W., Oreffo, R.O.C. and Dalby, M.J. (2009) Differential in-gel electrophoresis (DIGE) analysis of human bone marrow osteoprogenitor cell contact guidance. Acta Biomaterialia, 5(4), pp. 1137-1146. (doi:10.1016/j.actbio.2008.11.001)

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Publisher's URL: http://dx.doi.org/10.1016/j.actbio.2008.11.001

Abstract

We have used a recent comparative proteomics technique, differential in-gel electrophoresis (DIGE), to study osteoprogenitor cell response to contact guidance in grooves. In order to increase protein output from small sample sizes, we used bioreactor culture before protein extraction and gel electrophoresis. Mass spectroscopy was used for protein identification. A number of distinct proteins were observed to exhibit significant changes in expression. These changes in protein expression suggest that the cells respond to tailored grooved topographies, with alterations in their proteome concurrent with changes in osteoprogenitor phenotype

Item Type:Articles
Keywords:Tissue engineering, Surface topography, Contact guidance, Osteoprogenitor cells, Proteomics
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Burchmore, Dr Richard and Wilkinson, Professor Christopher and Dalby, Professor Matthew
Authors: Kantawong, F., Burchmore, R.J.S., Wilkinson, C.D.W., Oreffo, R.O.C., and Dalby, M.J.
Subjects:R Medicine > R Medicine (General)
Q Science > QP Physiology
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Acta Biomaterialia
ISSN:1742-7061

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