Proteomic analysis of human osteoprogenitor response to disordered nanotopography

Kantawong, F., Burchmore, R.J.S. , Gadegaard, N. , Oreffo, R.O.C. and Dalby, M.J. (2009) Proteomic analysis of human osteoprogenitor response to disordered nanotopography. Journal of the Royal Society: Interface, (doi:10.1098/rsif.2008.0447)

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Publisher's URL: http://dx.doi.org/10.1098/rsif.2008.0447

Abstract

Previous studies have shown that microgroove-initiated contact guidance can induce bone formation in osteoprogenitor cells (OPGs) and produce changes in the cell proteome. For proteomic analysis, differential in-gel electrophoresis (DIGE) can be used as a powerful diagnostic method to provide comparable data between the proteomic profiles of cells cultured in different conditions. This study focuses on the response of OPGs to a novel nanoscale pit topography with osteoinductive properties compared with planar controls. Disordered near-square nanopits with 120nm diameter and 100nm depth with an average 300nm centre-to-centre spacing (300nm spaced pits in square pattern, but with ±50nm disorder) were fabricated on 1+—1cm<sup>2</sup> polycaprolactone sheets. Human OPGs were seeded onto the test materials. DIGE analysis revealed changes in the expression of a number of distinct proteins, including upregulation of actin isoforms, beta-galectin1, vimentin and procollagen-proline, 2-oxoglutarate 4-dioxygenase and prolyl 4-hydroxylase. Downregulation of enolase, caldesmon, zyxin, GRASP55, Hsp70 (BiP/GRP78), RNH1, cathepsin D and Hsp27 was also observed. The differences in cell morphology and mineralization are also reported using histochemical techniques

Item Type:Articles
Keywords:nanotopography, nanobioscience, osteoprogenitor cells, tissue engineering, differential proteomics
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Burchmore, Dr Richard and Gadegaard, Professor Nikolaj and Dalby, Professor Matthew
Authors: Kantawong, F., Burchmore, R.J.S., Gadegaard, N., Oreffo, R.O.C., and Dalby, M.J.
Subjects:Q Science > QR Microbiology > QR180 Immunology
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Biomedical Engineering
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of the Royal Society: Interface
Journal Abbr.:J. R. Soc. Interface
ISSN:1742-5689
ISSN (Online):1742-5662
Published Online:09 December 2008

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