Metabolomic profiling using Orbitrap Fourier transform mass spectrometry with hydrophilic interaction chromatography: a method with wide applicability to analysis of biomolecules

Kamleh, A., Barrett, M.P. , Wildridge, D., Burchmore, R.J.S. , Scheltema, R.A.. and Watson, D.G. (2008) Metabolomic profiling using Orbitrap Fourier transform mass spectrometry with hydrophilic interaction chromatography: a method with wide applicability to analysis of biomolecules. Rapid Communications in Mass Spectrometry, 22(12), pp. 1912-1918. (doi:10.1002/rcm.3564)

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Publisher's URL: http://dx.doi.org/10.1002/rcm.3564

Abstract

It was shown that coupling hydrophilic interaction chromatography (HILIC) to Orbitrap Fourier transform mass spectrometery (FT-MS) provided an excellent tool for metabolic profiling, principally due to rapid elution of lipids in advance of most metabolites entering the mass spectrometer. We used <i>in vitro</i> cultivated procyclic forms of the protozoan parasite <i>Trypanosoma brucei</i> as a source of metabolites to test the performance of the HILIC column and the mass accuracy of MS. The mass accuracy achieved fell within 2ppm for all the metabolites identified within samples. It was, for example, possible to identify the signature metabolite of the trypanosome, trypanothione, and also glutathione which were well retained by the HILIC column. By comparing trypanosomes grown in two different media we were able to clearly distinguish the samples in terms of the relative abundance of a number of metabolites using Sieve 1.1 software.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Burchmore, Dr Richard and Barrett, Professor Michael
Authors: Kamleh, A., Barrett, M.P., Wildridge, D., Burchmore, R.J.S., Scheltema, R.A.., and Watson, D.G.
Subjects:Q Science > QR Microbiology > QR180 Immunology
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Rapid Communications in Mass Spectrometry
Publisher:John Wiley & Sons Ltd
ISSN:0951-4198
ISSN (Online):1097-0231
Published Online:09 May 2008
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
241291Molecular Genetic and Biochemical Analyses of ParasitesJeremy MottramMedical Research Council (MRC)G97222968Infection Immunity and Inflammation Life Sciences