The light intensity under which cells are grown controls the type of peripheral light-harvesting complexes that are assembled in a purple photosynthetic bacterium

Brotosudarmo, T.H.P., Collins, A.M., Gall, A., Roszak, A.W., Gardiner, A.T., Blankenship, R.E. and Cogdell, R.J. (2011) The light intensity under which cells are grown controls the type of peripheral light-harvesting complexes that are assembled in a purple photosynthetic bacterium. Biochemical Journal, 440(1), pp. 51-61. (doi:10.1042/BJ20110575)

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Abstract

The differing composition of LH2 (peripheral light-harvesting) complexes present in Rhodopseudomonas palustris 2.1.6 have been investigated when cells are grown under progressively decreasing light intensity. Detailed analysis of their absorption spectra reveals that there must be more than two types of LH2 complexes present. Purified HL (high-light) and LL (low-light) LH2 complexes have mixed apoprotein compositions. The HL complexes contain PucAB(a) and PucAB(b) apoproteins. The LL complexes contain PucAB(a), PucAB(d) and PucB(b)-only apoproteins. This mixed apoprotein composition can explain their resonance Raman spectra. Crystallographic studies and molecular sieve chromatography suggest that both the HL and the LL complexes are nonameric. Furthermore, the electron-density maps do not support the existence of an additional Bchl (bacteriochlorophyll) molecule; rather the density is attributed to the N-termini of the alpha-polypeptide.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Brotosudarmo, Dr Tatas Hardo and Cogdell, Professor Richard and Gardiner, Dr Alastair and Roszak, Dr Aleksander
Authors: Brotosudarmo, T.H.P., Collins, A.M., Gall, A., Roszak, A.W., Gardiner, A.T., Blankenship, R.E., and Cogdell, R.J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Chemistry
Journal Name:Biochemical Journal
ISSN:0264-6021
ISSN (Online):1470-8728
Published Online:28 July 2011

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