Generation of primary hepatocyte microarrays by piezoelectric printing

Zarowna-Dabrowska, A. et al. (2012) Generation of primary hepatocyte microarrays by piezoelectric printing. Colloids and Surfaces B: Biointerfaces, 89, pp. 126-132. (doi:10.1016/j.colsurfb.2011.09.016)

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Abstract

We demonstrate a single-step method for the generation of collagen and poly-l-Lysine (PLL) micropatterns on a poly(ethylene glycol) (PEG) functionalized glass surface for cell based assays. The method involves establishing a reliable silanization method to create an effective non-adhesive PEG layer on glass that inhibits cell attachment, followed by the spotting of collagen or PLL solutions using non-contact piezoelectric printing. We show for the first time that the spotted protein micropatterns remain stable on the PEG surface even after extensive washing, thus significantly simplifying protein pattern formation.

We found that adherence and spreading of NIH-3T3 fibroblasts was confined to PLL and collagen areas of the micropatterns. In contrast, primary rat hepatocytes adhered and spread only on collagen micropatterns, where they formed uniform, well defined functionally active cell arrays. The differing affinity of hepatocytes and NIH-3T3 fibroblasts for collagen and PLL patterns was used to develop a simple technique for creating a co-culture of the two cell types. This has the potential to form structured arrays that mimic the in vivo hepatic environment and is easily integrated within a miniaturized analytical platform for developing high throughput toxicity analysis in vitro.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Yin, Professor Huabing and Pitt, Dr Andrew and McKenna, Dr Ekaterina and Glidle, Dr Andrew and Cooper, Professor Jonathan
Authors: Zarowna-Dabrowska, A., McKenna, E.O., Schutte, M.E., Glidle, A., Chen, L., Cuestas-Ayllon, C., Marshall, D., Pitt, A., Dawson, M.D., Gu, E., Cooper, J.M., and Yin, H.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Colloids and Surfaces B: Biointerfaces
ISSN:0927-7765
Published Online:17 September 2011

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