Lack of effect of Miller Fisher sera/plasmas on transmitter release from PC12 cells

Benatar, M.G., Willison, H.J. and Vincent, A. (1997) Lack of effect of Miller Fisher sera/plasmas on transmitter release from PC12 cells. Journal of Neuroimmunology, 80(1-2), pp. 1-5. (doi: 10.1016/S0165-5728(97)00127-6)

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IgG antibodies to GQ<sub>1b</sub> ganglioside are found in > 90% of patients with the Miller Fisher Syndrome (MFS). MFS sera or IgG preparations have marked effects on neurotransmitter release at the neuromuscular junction, but their mode(s) of action remain unclear. To establish a cell-based system for investigating the mechanism of action of MFS serum preparations, we looked at neurotransmitter release from three cell lines. We failed to demonstrate substantial <sup>14</sup>C-acetylcholine release from two motor-neuronal cell lines, VSC4.1 and NSC19, and therefore studied <sup>3</sup>H-noradrenaline release from NGF-differentiated PC12 cells, a neural-crest derived catecholaminergic cell line. K<sup>+</sup>-induced release was inhibited by botulinum toxin and basal release was enhanced by alpha-latrotoxin, resembling that at the neuromuscular junction, although K<sup>+</sup>-induced release was dependent on L-type rather than P/Q-type calcium channels. The cells expressed polysialylated gangliosides on the cell surface. Incubation in heat-inactivated or untreated MFS preparations did not, however, affect basal or K<sup>+</sup>-induced release. Thus the PC12 cells do not appear to be sensitive to the effects of serum antibodies from MFS patients.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Willison, Professor Hugh
Authors: Benatar, M.G., Willison, H.J., and Vincent, A.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Journal of Neuroimmunology
ISSN (Online):1872-8421

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