Total ganglioside ablation at mouse motor nerve terminals alters neurotransmitter release level

Zitman, F.M.P., Todorov, B., Furukawa, K., Furukawa, K., Willison, H.J. and Plomp, J.J. (2010) Total ganglioside ablation at mouse motor nerve terminals alters neurotransmitter release level. Synapse, 64(4), pp. 335-338. (doi: 10.1002/syn.20747)

Abstract

Neuronal membrane gangliosides, forming a large family of sialylated glycosphingolipids, have been hypothesized to play important roles in synaptic transmission. We studied the ex vivo electrophysiological function of neuromuscular junctions of GM2/GD2-synthase*GD3-synthase compound null-mutant mice after acute removal of GM3, the only remaining ganglioside in this mouse, by in vitro treatment with neuraminidase. We found 16% enhancement of the acetylcholine release per nerve impulse at low-rate (0.3 Hz) nerve stimulation. Conversely, the treatment reduced the acetylcholine release evoked by high-rate (40 Hz) nerve stimulation. Also, 25 ms paired-pulse facilitation of endplate potentials was reduced by the neuraminidase-treatment. These effects may indicate a modest modulatory influence of the negative electrical charges carried by the sialic acid molecules of gangliosides on the function of presynaptic Ca_v2.1 channels, affecting the magnitude and kinetics of the Ca²⁺ influx that induces neurotransmitter release from the motor nerve terminal. Our results show that gangliosides are to some extent involved in neurotransmission at the neuromuscular junction, but that their presence is not an absolute requirement in this process.

• Item Type: Articles
• Additional Information: The definitive version is available at www3.interscience.wiley.com
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Willison, Professor Hugh
• Authors: Zitman, F.M.P., Todorov, B., Furukawa, K., Furukawa, K., Willison, H.J., and Plomp, J.J.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Synapse
• ISSN: 0887-4476
• ISSN (Online): 1098-2396
• Published Online: 02 December 2009
• Copyright Holders: Copyright © 2010 Wiley
• First Published: First published in Synapse 2010, 64(4):335-338
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher