Characterization of the anticollagen antibody response in a new model of chronic polyarthritis

Conigliaro, P., Benson, R. A. , Patakas, A., Kelly, S. M., Valesini, G., Holmdahl, R., Brewer, J. M. , McInnes, I. B. and Garside, P. (2011) Characterization of the anticollagen antibody response in a new model of chronic polyarthritis. Arthritis and Rheumatism, 63(8), pp. 2299-2308. (doi:10.1002/art.30413) (PMID:21506101)

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Abstract

Objective: Type II collagen (CII)-specific B cell responses have been recognized in human rheumatoid arthritis (RA) and in collagen-induced arthritis (CIA). An important limitation of the CIA model is that the CII response and the disease are stimulated by exogenously injected collagen. A model of experimental ovalbumin (OVA)-mediated acute arthritis has been established in which autoimmunity is spontaneous and elicited by antigen-specific T cells. This study was undertaken to create a new model of chronic autoimmune polyarthritis and characterize the associated CII-specific B cell response.<p></p> Methods: Secondary challenge with OVA or CII in adjuvant was used to elicit chronic disease. CII-specific B cell responses against the epitopes U1, J1, C1, and citrullinated C1, together with the antibody affinity, were investigated in OVA-mediated arthritis.<p></p> Results: Chronic autoimmune polyarthritis was induced and was dependent on the antigen used in the secondary challenge. U1 was the major CII epitope recognized, and antibodies showed the same affinity as those in CIA.<p></p> Conclusion: Our findings indicate that the development and severity of chronic disease is dependent on the antigen and is associated with an increased autoreactive B cell response directed against a specific CII epitope (U1). OVA-mediated chronic arthritis exhibits anti-CII antibodies (against U1), resembling human RA and murine CIA.<p></p>

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McInnes, Professor Iain and Conigliaro, Dr Paola and Patakas, Dr Agapitos and Benson, Dr Robert and Garside, Professor Paul and Brewer, Professor James
Authors: Conigliaro, P., Benson, R. A., Patakas, A., Kelly, S. M., Valesini, G., Holmdahl, R., Brewer, J. M., McInnes, I. B., and Garside, P.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Arthritis and Rheumatism
Publisher:Wiley
ISSN:0004-3591
ISSN (Online):2326-5205
Published Online:01 August 2011

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
497541DPFS Resource Request (University of Glasgow)Iain McInnesMedical Research Council (MRC)G0801687Infection Immunity and Inflammation Life Sciences