Screening trematodes for novel intervention targets: a proteomic and immunological comparison of Schistosoma haematobium, Schistosoma bovis and Echinostoma caproni

Higon, M. et al. (2011) Screening trematodes for novel intervention targets: a proteomic and immunological comparison of Schistosoma haematobium, Schistosoma bovis and Echinostoma caproni. Parasitology, 138(12), pp. 1607-1619. (doi:10.1017/S0031182011000412)

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Publisher's URL: http://dx.doi.org/10.1017/S0031182011000412

Abstract

With the current paucity of vaccine targets for parasitic diseases, particularly those in childhood, the aim of this study was to compare protein expression and immune cross-reactivity between the trematodes <i>Schistosoma haematobium</i>, <i>S. bovis</i> and <i>Echinostoma caproni</i> in the hope of identifying novel intervention targets. Native adult parasite proteins were separated by 2-dimensional gel electrophoresis and identified through electrospray ionisation tandem mass spectrometry to produce a reference gel. Proteins from differential gel electrophoresis analyses of the three parasite proteomes were compared and screened against sera from hamsters infected with <i>S. haematobium</i> and <i>E. caproni</i> following 2-dimensional Western blotting. Differential protein expression between the three species was observed with circa 5% of proteins from <i>S. haematobium</i> showing expression up-regulation compared to the other two species. There was 91% similarity between the proteomes of the two S <i>chistosoma</i> species and 81% and 78.6% similarity between <i>S. haematobium</i> and <i>S. bovis</i> versus <i>E. caproni</i>, respectively. Although there were some common cross-species antigens, species-species targets were revealed which, despite evolutionary homology, could be due to phenotypic plasticity arising from different host-parasite relationships. Nevertheless, this approach helps to identify novel intervention targets which could be used as broad-spectrum candidates for future use in human and veterinary vaccines.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Burchmore, Dr Richard
Authors: Higon, M., Cowan, G., Nausch, N., Cavanagh, D., Oleaga, A., Toledo, R., Stothard, J.R., Antunez, O., Marcilla, A., Burchmore, R.J.S., and Mutapi, F.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Parasitology
Publisher:Cambridge University Press
ISSN:0031-1820
ISSN (Online):1469-8161
Published Online:10 June 2011

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