Preventing and troubleshooting artefacts in saturation labelled fluorescence 2D difference gel electrophoresis (saturation DiGE)

McNamara, L.E., Kantawong, F., Dalby, M.J. , Riehle, M.O. and Burchmore, R.J.S. (2012) Preventing and troubleshooting artefacts in saturation labelled fluorescence 2D difference gel electrophoresis (saturation DiGE). Proteomics, 11(24), pp. 4610-4621. (doi: 10.1002/pmic.201100135)

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Publisher's URL: http://dx.doi.org/10.1002/pmic.201100135

Abstract

Saturation DiGE is a powerful but challenging technique for the characterisation of changes in protein expression between two or more scarce samples. In this paper, measures to prevent and troubleshoot artefacts in the saturation DiGE workflow are discussed, with illustration of some examples as they may be encountered in gel images or analysis.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Dalby, Professor Matthew and Riehle, Dr Mathis and Burchmore, Dr Richard and McNamara, Dr Laura
Authors: McNamara, L.E., Kantawong, F., Dalby, M.J., Riehle, M.O., and Burchmore, R.J.S.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
Journal Name:Proteomics
ISSN:1615-9853
ISSN (Online):1615-9861
Published Online:23 November 2011

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