Preventing and troubleshooting artefacts in saturation labelled fluorescence 2D difference gel electrophoresis (saturation DiGE)

McNamara, L.E., Kantawong, F., Dalby, M.J., Riehle, M.O. and Burchmore, R.J.S. (2012) Preventing and troubleshooting artefacts in saturation labelled fluorescence 2D difference gel electrophoresis (saturation DiGE). Proteomics, 11(24), pp. 4610-4621. (doi:10.1002/pmic.201100135)

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Publisher's URL: http://dx.doi.org/10.1002/pmic.201100135

Abstract

Saturation DiGE is a powerful but challenging technique for the characterisation of changes in protein expression between two or more scarce samples. In this paper, measures to prevent and troubleshoot artefacts in the saturation DiGE workflow are discussed, with illustration of some examples as they may be encountered in gel images or analysis.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Riehle, Dr Mathis and Burchmore, Dr Richard and McNamara, Dr Laura and Dalby, Professor Matthew
Authors: McNamara, L.E., Kantawong, F., Dalby, M.J., Riehle, M.O., and Burchmore, R.J.S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Proteomics
ISSN:1615-9853
ISSN (Online):1615-9861
Published Online:23 November 2011

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