Kitson, S.M., Mullen, W. , Cogdell, R.J. , Bill, R.M. and Fraser, N.J. (2012) GPCR production in a novel yeast strain that makes cholesterol-like sterols. Methods, 55(4), pp. 287-292. (doi: 10.1016/j.ymeth.2011.09.023) (PMID:22001123)
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Publisher's URL: http://dx.doi.org/10.1016/j.ymeth.2011.09.023
Abstract
The activities of many mammalian membrane proteins including G-protein coupled receptors are cholesterol-dependent. Unlike higher eukaryotes, yeast do not make cholesterol. Rather they make a related molecule called ergosterol. As cholesterol and ergosterol are biologically non-equivalent, the potential of yeast as hosts for overproducing mammalian membrane proteins has never been fully realised. To address this problem, we are trying to engineer a novel strain of Saccharomyces cerevisiae in which the cholesterol biosynthetic pathway of mammalian cells has been fully reconstituted. Thus far, we have created a modified strain that makes cholesterol-like sterols which has an increased capacity to make G-protein coupled receptors compared to control yeast.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Fraser, Dr Niall and Kitson, Miss Susan and Mullen, Dr Bill and Cogdell, Professor Richard |
Authors: | Kitson, S.M., Mullen, W., Cogdell, R.J., Bill, R.M., and Fraser, N.J. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | Methods |
ISSN: | 1046-2023 |
ISSN (Online): | 1095-9130) |
Published Online: | 06 October 2011 |
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