Forsythe, G. R., McCulloch, R. and Hammarton, T. C. (2009) Hydroxyurea-induced synchronisation of bloodstream stage Trypanosoma brucei. Molecular and Biochemical Parasitology, 164(2), pp. 131-136. (doi: 10.1016/j.molbiopara.2008.12.008)
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Publisher's URL: http://dx.doi.org/10.1016/j.molbiopara.2008.12.008
Abstract
Synchronisation of the <i>Trypanosoma brucei</i> cell cycle proved elusive for many years. A recent report demonstrated that synchronisation of procyclic form cells was possible following treatment with hydroxyurea. Here, that work is extended to the disease-relevant, mammalian-infective bloodstream stage trypanosome. Treatment of bloodstream stage Lister 427 <i>T. Brucei</i> cells growing <i>in vitro</i> with 10 μg ml<sup>−1</sup> hydroxyurea for 6 h led to an enrichment of cells in S phase. Following removal of the drug, cells proceeded uniformly through one round of the cell cycle, providing a much needed tool to enrich for specific cell cycle stages, in a manner similar to hydroxyurea treatment of procyclic form <i>T. brucei.</i>
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Hammarton, Dr Tansy and Forsythe, Mr Glynn |
| Authors: | Forsythe, G. R., McCulloch, R., and Hammarton, T. C. |
| Subjects: | Q Science > QL Zoology Q Science > QH Natural history > QH345 Biochemistry |
| College/School: | College of Medical Veterinary and Life Sciences College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Molecular and Biochemical Parasitology |
| Publisher: | Elsevier BV |
| ISSN: | 0166-6851 |
| ISSN (Online): | 1872-9428 |
| Published Online: | 27 December 2008 |
| Copyright Holders: | Copyright © 2009 Elsevier B.V. |
| First Published: | First published in Molecular and Biochemical Parasitology 164(2):131-136 |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
| Related URLs: |
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