Hydroxyurea-induced synchronisation of bloodstream stage Trypanosoma brucei

Forsythe, G. R., McCulloch, R. and Hammarton, T. C. (2009) Hydroxyurea-induced synchronisation of bloodstream stage Trypanosoma brucei. Molecular and Biochemical Parasitology, 164(2), pp. 131-136. (doi:10.1016/j.molbiopara.2008.12.008)

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Publisher's URL: http://dx.doi.org/10.1016/j.molbiopara.2008.12.008

Abstract

Synchronisation of the <i>Trypanosoma brucei</i> cell cycle proved elusive for many years. A recent report demonstrated that synchronisation of procyclic form cells was possible following treatment with hydroxyurea. Here, that work is extended to the disease-relevant, mammalian-infective bloodstream stage trypanosome. Treatment of bloodstream stage Lister 427 <i>T. Brucei</i> cells growing <i>in vitro</i> with 10 μg ml<sup>−1</sup> hydroxyurea for 6 h led to an enrichment of cells in S phase. Following removal of the drug, cells proceeded uniformly through one round of the cell cycle, providing a much needed tool to enrich for specific cell cycle stages, in a manner similar to hydroxyurea treatment of procyclic form <i>T. brucei.</i>

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Forsythe, Mr Glynn and Hammarton, Dr Tansy
Authors: Forsythe, G. R., McCulloch, R., and Hammarton, T. C.
Subjects:Q Science > QL Zoology
Q Science > QH Natural history > QH345 Biochemistry
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Molecular and Biochemical Parasitology
Publisher:Elsevier BV
ISSN:0166-6851
ISSN (Online):1872-9428
Published Online:27 December 2008
Copyright Holders:Copyright © 2009 Elsevier B.V.
First Published:First published in Molecular and Biochemical Parasitology 164(2):131-136
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
401101DNA recombination pathways and antigenic variation in trypanosoma bruceiRichard McCullochMedical Research Council (MRC)G0401553Infection Immunity and Inflammation Life Sciences
403591Regulation of cytokinesis in trypanosoma bruceiTansy HammartonMedical Research Council (MRC)G120/1001Infection Immunity and Inflammation Life Sciences