Hydroxyurea-induced synchronisation of bloodstream stage Trypanosoma brucei

Forsythe, G. R., McCulloch, R. and Hammarton, T. C. (2009) Hydroxyurea-induced synchronisation of bloodstream stage Trypanosoma brucei. Molecular and Biochemical Parasitology, 164(2), pp. 131-136. (doi: 10.1016/j.molbiopara.2008.12.008)

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Publisher's URL: http://dx.doi.org/10.1016/j.molbiopara.2008.12.008

Abstract

Synchronisation of the <i>Trypanosoma brucei</i> cell cycle proved elusive for many years. A recent report demonstrated that synchronisation of procyclic form cells was possible following treatment with hydroxyurea. Here, that work is extended to the disease-relevant, mammalian-infective bloodstream stage trypanosome. Treatment of bloodstream stage Lister 427 <i>T. Brucei</i> cells growing <i>in vitro</i> with 10 μg ml<sup>−1</sup> hydroxyurea for 6 h led to an enrichment of cells in S phase. Following removal of the drug, cells proceeded uniformly through one round of the cell cycle, providing a much needed tool to enrich for specific cell cycle stages, in a manner similar to hydroxyurea treatment of procyclic form <i>T. brucei.</i>

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hammarton, Dr Tansy and Forsythe, Mr Glynn
Authors: Forsythe, G. R., McCulloch, R., and Hammarton, T. C.
Subjects:Q Science > QL Zoology
Q Science > QH Natural history > QH345 Biochemistry
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Molecular and Biochemical Parasitology
Publisher:Elsevier BV
ISSN:0166-6851
ISSN (Online):1872-9428
Published Online:27 December 2008
Copyright Holders:Copyright © 2009 Elsevier B.V.
First Published:First published in Molecular and Biochemical Parasitology 164(2):131-136
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher
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