Gentamicin-attenuated Leishmania infantum: A clinicopathological study in dogs

Daneshvar, H., Molaei, M.M., Afshar, R.M., Kamiabi, H., Burchmore, R.J.S., Hagan, P. and Phillips, R.S. (2009) Gentamicin-attenuated Leishmania infantum: A clinicopathological study in dogs. Veterinary Immunology and Immunopathology, 129(1-2), pp. 28-35. (doi:10.1016/j.vetimm.2008.12.002)

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Publisher's URL: http://dx.doi.org/10.1016/j.vetimm.2008.12.002

Abstract

The clinicopathological changes following infection with an attenuated line of Leishmania infantum (L. infantum H-line) were evaluated in mixed breed dogs. Two groups of dogs were infected intravenously (i.v.) or intradermally (i.d.) with L. infantum H-line and two control groups were infected i.v. or i.d. with L. infantum wild-type (L. infantum WT). None of the dogs, which were infected i.v. or i.d. with L. infantum H-line, showed any abnormalities during the observation period. In contrast, two out of three dogs, which were infected i.v. with L. infantum WT, developed clinical signs of disease. In addition, no histopathological changes were seen in the liver and spleen of the dogs infected with the attenuated line of parasite, whereas the histopathological changes in the two dogs infected i.v. with L. infantum WT were severe in form and manifested by infiltration of high numbers of inflammatory cells. No promastigotes were found in cultures set up from spleens and livers of dogs infected with L. infantum H-line at 12 months post-infection, whereas promastigotes were seen in the spleen and liver cultures from 2 dogs infected i.v. with L. infantum WT. Serum levels of total IgG anti-Leishmania antibody were raised in all dogs. The antibody level in the serum of dogs infected i.v. with L. infantum WT was higher than that in dogs infected with L. infantum H-line. These results show no clinicopathological abnormalities in the dogs infected with gentamicin-attenuated L. infantum H-line. Moreover, L. infantum H-line induced IgG anti-Leishmania antibody in the dogs.

Item Type:Articles
Keywords:<i>Leishmania infantum</i>; gentamicin attenuated line; vaccine; safety; dog
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hagan, Professor Paul and Burchmore, Dr Richard and Phillips, Professor Stephen
Authors: Daneshvar, H., Molaei, M.M., Afshar, R.M., Kamiabi, H., Burchmore, R.J.S., Hagan, P., and Phillips, R.S.
Subjects:Q Science > QR Microbiology > QR355 Virology
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Veterinary Immunology and Immunopathology
ISSN:0165-2427

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