Cumming, S.A., Chuen-Im, T., Zhang, J. and Graham, S.V. (2009) The RNA stability regulator HuR regulates L1 protein expression in vivo in differentiating cervical epithelial cells. Virology, 383(1), pp. 142-149. (doi: 10.1016/j.virol.2008.10.003)
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Publisher's URL: http://dx.doi.org/10.1016/j.virol.2008.10.003
Abstract
Human papillomavirus (HPV) L1 and L2 capsid protein expression is restricted to the granular layer of infected, stratified epithelia and is regulated at least partly at post-transcriptional levels. For HPV16, a 79 nt late regulatory element (LRE) is involved in this control. Using W12 cells as a model for HPV16-infected differentiating cervical epithelial cells we show that HuR, a key cellular protein that controls mRNA stability, binds the LRE most efficiently in nuclear and cytoplasmic extracts of differentiated cells. Further, HuR binds the 3' U-rich portion of the LRE directly in vitro. Overexpression of HuR in undifferentiated W12 cells results in an increase in L1 mRNA and protein levels while siRNA knock-down of HuR in differentiated W12 cells depletes L1 expression. In differentiated cervical epithelial cells HuR may bind and stabilise L1 mRNAs aiding translation of L1 protein
Item Type: | Articles |
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Keywords: | HPV16, late gene expression, late regulatory element, HuR, epithelial differentiation |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Graham, Professor Sheila |
Authors: | Cumming, S.A., Chuen-Im, T., Zhang, J., and Graham, S.V. |
Subjects: | Q Science > QR Microbiology > QR355 Virology Q Science > QR Microbiology |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
Journal Name: | Virology |
ISSN: | 0042-6822 |
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