Imaging molecular dynamics in vivo - from cell biology to animal models

Timpson, P., McGhee, E.J. and Anderson, K.I. (2011) Imaging molecular dynamics in vivo - from cell biology to animal models. Journal of Cell Science, 124(17), pp. 2877-2890. (doi: 10.1242/jcs.085191)

Abstract

Advances in fluorescence microscopy have enabled the study of membrane diffusion, cell adhesion and signal transduction at the molecular level in living cells grown in culture. By contrast, imaging in living organisms has primarily been restricted to the localization and dynamics of cells in tissues. Now, imaging of molecular dynamics is on the cusp of progressing from cell culture to living tissue. This transition has been driven by the understanding that the microenvironment critically determines many developmental and pathological processes. Here, we review recent progress in fluorescent protein imaging <i>in vivo</i> by drawing primarily on cancer-related studies in mice. We emphasize the need for techniques that can be easily combined with genetic models and complement fluorescent protein imaging by providing contextual information about the cellular environment. In this commentary we will consider differences between <i>in vitro</i> and <i>in vivo</i> experimental design and argue for an approach to <i>in vivo</i> imaging that is built upon the use of intermediate systems, such as 3-D and explant culture models, which offer flexibility and control that is not always available <i>in vivo</i>. Collectively, these methods present a paradigm shift towards the molecular-level investigation of disease and therapy in animal models of disease.

• Item Type: Articles
• Keywords: In vivo imaging, intravital imaging, FRAP, FRET, FLIM, GPP, SHG
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Anderson, Professor Kurt and McGhee, Dr Ewan and Timpson, Dr Paul
• Authors: Timpson, P., McGhee, E.J., and Anderson, K.I.
• Subjects: Q Science > QH Natural history > QH301 Biology
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Journal of Cell Science
• ISSN: 0021-9533
• ISSN (Online): 1477-9137