pH and carbon supply control the expression of phosphoenolpyruvate carboxylase kinase genes in Arabidopsis thaliana

Chen, Z.-H., Jenkins, G.I. and Nimmo, H.G. (2008) pH and carbon supply control the expression of phosphoenolpyruvate carboxylase kinase genes in Arabidopsis thaliana. Plant, Cell and Environment, 31(12), pp. 1844-1850. (doi:10.1111/j.1365-3040.2008.01885.x) (PMID:18771573)

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Abstract

Phosphoenolpyruvate carboxylase (PEPC) is thought to play many roles in C<sub>3</sub> plants including the provision of biosynthetic precursors and control of pH during N assimilation. Its activity is controlled via phosphorylation catalysed by PEPC kinases, which are encoded by <i>PPCK</i> genes. We examined <i>PPCK</i> expression in response to changes in the supply of N or C, and to changes in intracellular pH, using cultured Arabidopsis cells and seedlings. The results show that expression of both <i>PPCK1</i> and <i>PPCK2</i> is increased by C availability, but does not respond to N availability. Expression of the two <i>PPCK</i> genes and the phosphorylation state of PEPC are increased in response to increasing intracellular pH. Elevated pH also reduces the repression of <i>PPCK</i> gene expression by P<sub>i</sub>. Expression of phosphoenolpyruvate carboxykinase (PEPCK), which catalyses the decarboxylation of oxaloacetate, is decreased in response to increasing intracellular pH. pH homeostasis may be mediated at least partly by reciprocal changes in the expression of <i>PPCK</i> genes and PEPCK.

Item Type:Articles
Additional Information:A correction to this article is available at https://doi.org/10.1111/j.1365-3040.2008.01924.x.
Keywords:Intracellular pH, nitrogen supply, phosphoenolpyruvate carboxykinase, protein phosphorylation.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Jenkins, Professor Gareth and Chen, Dr Zhi-Hui and Nimmo, Professor Hugh
Authors: Chen, Z.-H., Jenkins, G.I., and Nimmo, H.G.
Subjects:Q Science > QH Natural history > QH345 Biochemistry
Q Science > QP Physiology
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Plant, Cell and Environment
ISSN:0140-7791
ISSN (Online):1365-3040
Published Online:02 September 2008
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