Influence of both a static magnetic field and penetratin on magnetic nanoparticle delivery into fibroblasts

Dejardin, T., de la Fuente, J., del Pino, P., Furlani, E.P., Mullin, M., Smith, C.A. and Berry, C.C. (2012) Influence of both a static magnetic field and penetratin on magnetic nanoparticle delivery into fibroblasts. Nanomedicine, 6(10), pp. 1719-1731. (doi: 10.2217/nnm.11.65)

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Abstract

Aim: With regards to nanoparticles, all biomedical applications require cellular uptake, which to date remains a hurdle to further progress. This study aims to compare both the attractive force of a static magnetic field and the cell penetrating capability of penetratin; two techniques currently employed to enhance cell uptake. Materials and Methods: Fluorescent magnetic nanoparticles were functionalized with penetratin and cells were challenged with or without the particles in the presence/absence of a static magnetic field (350 mT). Following analysis of the magnetic field applied, cellular uptake and behavior was assessed in terms of fluorescence microscopy, clathrin and caveolin levels, scanning electron microscopy and transmission electron microscopy. Results: Modeling of the field applied demonstrated varying field patterns across the cell culture area, reflected by higher particle uptake at higher field strengths. Both penetratin and the magnetic field increased cell uptake with penetratin proving more efficient. Interestingly, the magnetic field stimulated clathrin-mediated endocytosis and subsequent particle uptake.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Berry, Dr Catherine and Smith, Mrs Carol-Anne
Authors: Dejardin, T., de la Fuente, J., del Pino, P., Furlani, E.P., Mullin, M., Smith, C.A., and Berry, C.C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Nanomedicine
ISSN:1743-5889
Published Online:02 August 2011

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