Investigation into omacetaxine solution stability for in vitro study

Marenah, L., Allan, E.K., Mountford, J.C., Holyoake, T.L., Jorgensen, H.G. and Elliott, M.A. (2012) Investigation into omacetaxine solution stability for in vitro study. Biomedical Chromatography, 26(5), pp. 545-547. (doi:10.1002/bmc.1686)

Marenah, L., Allan, E.K., Mountford, J.C., Holyoake, T.L., Jorgensen, H.G. and Elliott, M.A. (2012) Investigation into omacetaxine solution stability for in vitro study. Biomedical Chromatography, 26(5), pp. 545-547. (doi:10.1002/bmc.1686)

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Abstract

Omacetaxine is a natural product extract originating from Chinese medicine and finding therapeutic use as a potent myelosuppressive agent in leukemia. When planning <i>in vitro</i> cell biology experiments to assess omacetaxine activity against primary leukemic stem cells, it became apparent that the literature rarely describes the <i>in vitro</i> stability of the molecule, although accessible chromatographic methods have been published. Clearly whole organisms vs their component cells will differ in the way in which they handle xenobiotics, with the latter more dependent on physiochemical parameters such as pH and temperature in the absence of active metabolism or excretion. This could impact on the cells' experience of drug in culture. We therefore report here on examination of a modified, high-performance liquid chromatography (HPLC) method with assessment of degradant production from a 72 h solution stability study, clearly demonstrating that omacetaxine is highly stable in representative cell culture conditions (37 °C, neutral pH) and persists for many days in marked contrast to its short-half life <i>in vivo</i>.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Holyoake, Professor Tessa and Mountford, Dr Joanne and Jorgensen, Dr Heather and Marenah, Dr Lamin and Allan, Mrs Elaine
Authors: Marenah, L., Allan, E.K., Mountford, J.C., Holyoake, T.L., Jorgensen, H.G., and Elliott, M.A.
Subjects:Q Science > QP Physiology
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
College of Medical Veterinary and Life Sciences > Institute of Neuroscience and Psychology
Journal Name:Biomedical Chromatography
ISSN:0269-3879
ISSN (Online):1099-0801
Published Online:09 August 2011

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