Nanoscale surfaces for the long-term maintenance of mesenchymal stem cell phenotype and multipotency

McMurray, R.J., Gadegaard, N. , Tsimbouri, P.M. , Burgess, K.V., McNamara, L.E., Tare, R., Murawski, K., Kingham, E., Oreffo, R.O.C. and Dalby, M.J. (2011) Nanoscale surfaces for the long-term maintenance of mesenchymal stem cell phenotype and multipotency. Nature Materials, 10(8), pp. 637-644. (doi:10.1038/nmat3058)

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Abstract

There is currently an unmet need for the supply of autologous, patient-specific stem cells for regenerative therapies in the clinic. Mesenchymal stem cell differentiation can be driven by the material/cell interface suggesting a unique strategy to manipulate stem cells in the absence of complex soluble chemistries or cellular reprogramming. However, so far the derivation and identification of surfaces that allow retention of multipotency of this key regenerative cell type have remained elusive. Adult stem cells spontaneously differentiate in culture, resulting in a rapid diminution of the multipotent cell population and their regenerative capacity. Here we identify a nanostructured surface that retains stem-cell phenotype and maintains stem-cell growth over eight weeks. Furthermore, the study implicates a role for small RNAs in repressing key cell signalling and metabolomic pathways, demonstrating the potential of surfaces as non-invasive tools with which to address the stem cell niche.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Tsimbouri, Dr Penelope and Dalby, Professor Matthew and Burgess, Dr Karl and McNamara, Dr Laura and Gadegaard, Professor Nikolaj
Authors: McMurray, R.J., Gadegaard, N., Tsimbouri, P.M., Burgess, K.V., McNamara, L.E., Tare, R., Murawski, K., Kingham, E., Oreffo, R.O.C., and Dalby, M.J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Nature Materials
Publisher:Nature Publishing Group
ISSN:1476-1122
ISSN (Online):1476-4660
Published Online:17 July 2011

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
431761Optimising nanodisorder for bone tissue engineeringMatthew DalbyBiotechnology and Biological Sciences Research Council (BBSRC)BB/E526015/1RI MOLECULAR CELL & SYSTEMS BIOLOGY