Expansion in scid mice of Epstein-Barr virus-associated post-transplantation lymphoproliferative disease biopsy material

Johannessen, I., Perera, S.M., Gallagher, A., Hopwood, P.A., Thomas, J.A. and Crawford, D.H. (2002) Expansion in scid mice of Epstein-Barr virus-associated post-transplantation lymphoproliferative disease biopsy material. Journal of General Virology, 83(1), pp. 173-178.

Full text not currently available from Enlighten.

Publisher's URL: http://vir.sgmjournals.org/content/83/1/173.abstract

Abstract

Post-transplant lymphoproliferative disease (PTLD) biopsy material is rarely available in adequate quantity for research. Therefore, the present study was designed to expand biopsy material in scid mice. Epstein-Barr virus (EBV)+ve PTLD samples from five transplant patients were established in scid mice. PCR analysis of immunoglobulin gene rearrangements demonstrated that four of the five biopsies (80%) gave rise to scid tumours which represented the original tumour cell clones. Immunophenotyping showed that these four biopsies (and all scid tumours) expressed all EBV latent genes and a B lymphoblast phenotype; <or=26% T cells were found in the biopsy material whereas scid tumours showed a paucity of T lymphocytes. RT-PCR analysis revealed expression of IL-2, -4, -6, -10 and IFN-gamma in all tumour material, suggesting key roles for these factors in tumour growth. The results show that EBV+ve PTLD material can be expanded in scid mice giving rise to quantities of homogeneous malignant tissue sufficient for research studies.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Gallagher, Dr Alice
Authors: Johannessen, I., Perera, S.M., Gallagher, A., Hopwood, P.A., Thomas, J.A., and Crawford, D.H.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of General Virology
Publisher:Society for General Microbiology
ISSN:0022-1317
Related URLs:

University Staff: Request a correction | Enlighten Editors: Update this record