Cell chip array for microfluidic proteomics enabling rapid in situ assessment of intracellular protein phosphorylation

Faley, S.L., Copland, M. , Reboud, J. and Cooper, J.M. (2011) Cell chip array for microfluidic proteomics enabling rapid in situ assessment of intracellular protein phosphorylation. Biomicrofluidics, 5(2), 024106. (doi:10.1063/1.3587095)

Full text not currently available from Enlighten.

Publisher's URL: http://dx.doi.org/10.1063/1.3587095

Abstract

We discuss the ability to perform fluorescent immunocytochemistry, following cell fixation, using a microfluidic array of primary, nonadherent, single CD34+ stem cells. The technique requires small cell samples and proceeds with no cell loss, making it well-suited to monitoring these rare patient-derived cells. The chip allows us to correlate live cell dynamics across arrays of individual cells with post-translational modifications of intracellular proteins, following their exposure to drug treatments. Results also show that due to the microfluidic environment, the time scale of cell fixation was significantly reduced compared to conventional methods, leading to greater confidence in the status of the protein modifications studied. (C) 2011 American Institute of Physics.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cooper, Professor Jonathan and Reboud, Dr Julien and Faley, Dr Shannon and Copland, Professor Mhairi
Authors: Faley, S.L., Copland, M., Reboud, J., and Cooper, J.M.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Biomicrofluidics
Publisher:American Institute of Physics
ISSN:1932-1058

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
UNSPECIFIEDUNSPECIFIEDUNSPECIFIEDUNSPECIFIEDUNSPECIFIEDG84/6317UNSPECIFIED