Validation of the Cozart^® microplate EIA for analysis of opiates in oral fluid

Cooper, G., Wilson, L., Reid, C., Baldwin, D., Hand, C. and Spiehler, V. (2005) Validation of the Cozart^® microplate EIA for analysis of opiates in oral fluid. Forensic Science International, 154(2-3), pp. 240-246. (doi: 10.1016/j.forsciint.2004.12.042)

Abstract

The purpose of this study was to determine the performance characteristics of the Cozart^® microplate EIA for detecting opiates in oral fluid from patients in a drug misuse treatment program. Oral fluid samples were collected using the Cozart^® RapiScan Collection System from 216 donors who were receiving treatment for their addiction and were monitored for drug abuse. A further 40 oral fluid samples were collected from volunteer donors who were not drug users. The samples were analyzed in the laboratory by using the Cozart^® microplate EIA for opiates and confirmed using gas chromatography–mass spectrometry (GC–MS). The samples were stored frozen until analysis by GC–MS. The intra-assay precision for the Cozart^® microplate oral fluid EIA for opiates over 40 assays was 0.43% to 9.13% CV (within assay) and 2.9% to 9.1% CV (within day). A total of 109 samples were positive for various opiates. The Cozart® microplate EIA for opiates in oral fluid, using a cut-off of 30 ng/ml morphine equivalents in neat oral fluid, had a sensitivity of 99.1 ± 2.1% and a specificity of 94.4 ± 2.2% versus GC–MS. A series of potential adulterants of oral fluid were evaluated and shown not to alter the outcome of the test result.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Cooper, Dr Gail
• Authors: Cooper, G., Wilson, L., Reid, C., Baldwin, D., Hand, C., and Spiehler, V.
• Subjects: R Medicine > RA Public aspects of medicine > RA1001 Forensic Medicine. Medical jurisprudence. Legal medicine
• College/School: College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Clinical Specialities
• Journal Name: Forensic Science International
• Publisher: Elsevier Ireland Ltd
• ISSN: 0379-0738
• ISSN (Online): 1872-6283
• Published Online: 15 December 2004