Faecal microbiota and short chain fatty acid levels in faeces from infants with cow's milk protein allergy

Thompson-Chagoyan, O.C., Fallani, M., Maldonado, J., Vieties, J.M., Khanna, S., Edwards, C. , Dore, J. and Gil, A. (2011) Faecal microbiota and short chain fatty acid levels in faeces from infants with cow's milk protein allergy. International Archives of Allergy and Immunology, 156(3), pp. 325-332. (doi:10.1159/000323893)

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Abstract

The present study was designed to compare the faecal microbiota and concentrations of faecal short chain fatty acid and ammonia between healthy and cow's milk protein allergic (CMPA) infants. Methods:The population comprised 92 infants aged 2-12 months who were nonallergic (n=46) or diagnosed as having CMPA (n=46). Faecal samples were analysed by fluorescent in situ hybridization and flow cytometry using a panel of 10 rRNA targeted group and species specific oligonucleotide probes. Acetic, propionic, butyric, isocarpoic and branched chain short chain fatty acids (BCSFA)were measured by gas-liquid chromatography, lactate by enzymic reastion and pH and ammonia levels were determined. Results: CMPA infant faeces had significantly higher proportions of the Clostridium coccoides group and Atopobium cluster and a higher sum of the proportions of the different bacterial groups in comparison to healthy infant faeces. Faecal pH and ammonia did not significantly differ between CPMA and healthy infants. Faeces concentrations and percentages of butyric acid and BCSFA were higher in CMPA infants than in healthy infants. Conclusions: The findings clearly set a link between a dysbiosis in gut microbiota composition and the pathogenesis of CMPA. No single species or genus appeared to play an essential role, but dysbiosis led to biomarkers of CMPA among bacterial fermentation products.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Shepherd, Dr Sheila and Edwards, Professor Christine
Authors: Thompson-Chagoyan, O.C., Fallani, M., Maldonado, J., Vieties, J.M., Khanna, S., Edwards, C., Dore, J., and Gil, A.
Subjects:R Medicine > R Medicine (General)
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Centre for Population and Health Sciences
Journal Name:International Archives of Allergy and Immunology
Publisher:S. Karger AG
ISSN:1018-2438
ISSN (Online):1423-0097
Published Online:29 June 2011

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
326351INFABIO - Effect of diet & Lifestyle on risk of gastrointestinal infection & allergy in early life; consumer knowledge, attitudes and needsChristine EdwardsEuropean Commission (EC)QLK1-CT-2002-02Life-course Nutrition and Health