Grooved surface topography alters matrix-metalloproteinase production by human fibroblasts

Brydone, A. S., Dalby, M. J. , Berry, C. C. , Dominic Meek, R.M. D. and McNamara, L. E. (2011) Grooved surface topography alters matrix-metalloproteinase production by human fibroblasts. Biomedical Materials, 6(3), 035005. (doi:10.1088/1748-6041/6/3/035005)

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Publisher's URL: http://dx.doi.org/10.1088/1748-6041/6/3/035005

Abstract

Extracellular matrix (ECM) remodelling is an essential physiological process in which matrix-metalloproteinases (MMPs) have a key role. Manipulating the manner in which cells produce MMPs and ECMs may enable the creation of a desired tissue type, i.e. effect repair, or the prevention of tissue invasion (e.g. metastasis). The aim of this project was to determine if culturing fibroblasts on grooved topography altered collagen deposition or MMP production. Human fibroblasts were seeded on planar or grooved polycaprolactone substrates (grooves were 12.5 µm wide with varying depths of 240 nm, 540 nm or 2300 nm). Cell behaviour and collagen production were studied using fluorescence microscopy and the spent culture medium was assessed using gel zymography to detect MMPs. Total collagen deposition was high on the 240 nm deep grooves, but decreased as the groove depth increased, i.e. as cell contact guidance decreased. There was an increase in gelatinase on the 2300 nm deep grooved topography and there was a difference in the temporal expression of MMP-3 observed on the planar surface compared to the 540 nm and 2300 nm topographies. These results show that topography can alter collagen and MMP production. A fuller understanding of these processes may permit the design of surfaces tailored to tissue regeneration e.g. tendon repair.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McNamara, Dr Laura and Berry, Dr Catherine and Dalby, Professor Matthew
Authors: Brydone, A. S., Dalby, M. J., Berry, C. C., Dominic Meek, R.M. D., and McNamara, L. E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Biomedical Materials
Publisher:IOP Publishing
ISSN:1748-6041
ISSN (Online):1748-605X
Published Online:20 April 2011

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
503711Epithelial stress sensors: novel roles for cytochrome P450s and organellar calcium in integrated stress and immune responses.Shireen DaviesBiotechnology and Biological Sciences Research Council (BBSRC)BB/G020620/1Institute of Molecular Cell and Systems Biology
502891Micro- and nano-patterning of titanium surfaces for optimal osseointegration of orthopaedic implantsMatthew DalbyEngineering & Physical Sciences Research Council (EPSRC)EP/G048703/1Institute of Molecular Cell and Systems Biology