Measurement of H2O2 within living drosophila during aging using a ratiometric mass spectrometry probe targeted to the mitochondrial matrix

Cochemé, H.M. et al. (2011) Measurement of H2O2 within living drosophila during aging using a ratiometric mass spectrometry probe targeted to the mitochondrial matrix. Cell Metabolism, 13(3), pp. 340-350. (doi:10.1016/j.cmet.2011.02.003) (PMID:21356523) (PMCID:PMC4413513)

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Abstract

Hydrogen peroxide (H2O2) is central to mitochondrial oxidative damage and redox signaling, but its roles are poorly understood due to the difficulty of measuring mitochondrial H2O2 in vivo. Here we report a ratiometric mass spectrometry probe approach to assess mitochondrial matrix H2O2 levels in vivo. The probe, MitoB, comprises a triphenylphosphonium (TPP) cation driving its accumulation within mitochondria, conjugated to an arylboronic acid that reacts with H2O2 to form a phenol, MitoP. Quantifying the MitoP/MitoB ratio by liquid chromatography-tandem mass spectrometry enabled measurement of a weighted average of mitochondrial H2O2 that predominantly reports on thoracic muscle mitochondria within living flies. There was an increase in mitochondrial H2O2 with age in flies, which was not coordinately altered by interventions that modulated life span. Our findings provide approaches to investigate mitochondrial ROS in vivo and suggest that while an increase in overall mitochondrial H2O2 correlates with aging, it may not be causative.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hartley, Professor Richard
Authors: Cochemé, H.M., Quin, C., McQuaker, S.J., Cabreiro, F., Logan, A., Prime, T.A., Abakumova, I., Patel, J.V., Fearnley, I.M., James, A.M., Porteous, C.M., Smith, R.A.J., Saeed, S., Carré, J.E., Singer, M., Gems, D., Hartley, R.C., Partridge, L., and Murphy, M.P.
Subjects:Q Science > QD Chemistry
College/School:College of Science and Engineering > School of Chemistry
Journal Name:Cell Metabolism
Journal Abbr.:Cell Metab.
Publisher:Elsevier Inc.
ISSN:1550-4131
ISSN (Online):1932-7420
Published Online:01 January 2011
Copyright Holders:Copyright © 2011 Elsevier Inc.
First Published:First published in Cell Metabolism 13(3):340-350
Publisher Policy:Reproduced under a Creative Commons License

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