Cochemé, H.M. et al. (2011) Measurement of H2O2 within living drosophila during aging using a ratiometric mass spectrometry probe targeted to the mitochondrial matrix. Cell Metabolism, 13(3), pp. 340-350. (doi: 10.1016/j.cmet.2011.02.003) (PMID:21356523) (PMCID:PMC4413513)
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Abstract
Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is central to mitochondrial oxidative damage and redox signaling, but its roles are poorly understood due to the difficulty of measuring mitochondrial H<sub>2</sub>O<sub>2</sub> in vivo. Here we report a ratiometric mass spectrometry probe approach to assess mitochondrial matrix H<sub>2</sub>O<sub>2</sub> levels in vivo. The probe, MitoB, comprises a triphenylphosphonium (TPP) cation driving its accumulation within mitochondria, conjugated to an arylboronic acid that reacts with H<sub>2</sub>O<sub>2</sub> to form a phenol, MitoP. Quantifying the MitoP/MitoB ratio by liquid chromatography-tandem mass spectrometry enabled measurement of a weighted average of mitochondrial H<sub>2</sub>O<sub>2</sub> that predominantly reports on thoracic muscle mitochondria within living flies. There was an increase in mitochondrial H<sub>2</sub>O<sub>2</sub> with age in flies, which was not coordinately altered by interventions that modulated life span. Our findings provide approaches to investigate mitochondrial ROS in vivo and suggest that while an increase in overall mitochondrial H<sub>2</sub>O<sub>2</sub> correlates with aging, it may not be causative.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Hartley, Professor Richard |
| Authors: | Cochemé, H.M., Quin, C., McQuaker, S.J., Cabreiro, F., Logan, A., Prime, T.A., Abakumova, I., Patel, J.V., Fearnley, I.M., James, A.M., Porteous, C.M., Smith, R.A.J., Saeed, S., Carré, J.E., Singer, M., Gems, D., Hartley, R.C., Partridge, L., and Murphy, M.P. |
| Subjects: | Q Science > QD Chemistry |
| College/School: | College of Science and Engineering > School of Chemistry |
| Journal Name: | Cell Metabolism |
| Journal Abbr.: | Cell Metab. |
| Publisher: | Elsevier Inc. |
| ISSN: | 1550-4131 |
| ISSN (Online): | 1932-7420 |
| Published Online: | 01 January 2011 |
| Copyright Holders: | Copyright © 2011 Elsevier Inc. |
| First Published: | First published in Cell Metabolism 13(3):340-350 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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