Stroke penumbra defined by an MRI-based oxygen challenge technique: 1. validation using [14C]2-deoxyglucose autoradiography

Robertson, C. A., McCabe, C. , Gallagher, L., Lopez-Gonzalez, M. d., Holmes, W. M. , Condon, B., Muir, K. W. , Santosh, C. and Macrae, I. M. (2011) Stroke penumbra defined by an MRI-based oxygen challenge technique: 1. validation using [14C]2-deoxyglucose autoradiography. Journal of Cerebral Blood Flow and Metabolism, 31, pp. 1778-1787. (doi:10.1038/jcbfm.2011.66)

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Publisher's URL: http://dx.doi.org/10.1038/jcbfm.2011.66

Abstract

Accurate identification of ischemic penumbra will improve stroke patient selection for reperfusion therapies and clinical trials. Current magnetic resonance imaging (MRI) techniques have limitations and lack validation. Oxygen challenge T2* MRI (T2* OC) uses oxygen as a biotracer to detect tissue metabolism, with penumbra displaying the greatest T2* signal change during OC. [14C]2-deoxyglucose (2-DG) autoradiography was combined with T2* OC to determine metabolic status of T2*-defined penumbra. Permanent middle cerebral artery occlusion was induced in anesthetized male Sprague-Dawley rats (n=6). Ischemic injury and perfusion deficit were determined by diffusion- and perfusion-weighted imaging, respectively. At 147±32 minutes after stroke, T2* signal change was measured during a 5-minute 100% OC, immediately followed by 125 μCi/kg 2-DG, intravenously. Magnetic resonance images were coregistered with the corresponding autoradiograms. Regions of interest were located within ischemic core, T2*-defined penumbra, equivalent contralateral structures, and a region of hyperglycolysis. A T2* signal increase of 9.22%±3.9% (mean±s.d.) was recorded in presumed penumbra, which displayed local cerebral glucose utilization values equivalent to contralateral cortex. T2* signal change was negligible in ischemic core, 3.2%±0.78% in contralateral regions, and 1.41%±0.62% in hyperglycolytic tissue, located outside OC-defined penumbra and within the diffusion abnormality. The results support the utility of OC-MRI to detect viable penumbral tissue followi

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Gallagher, Mrs Lindsay and Macrae, Professor I Mhairi and Condon, Professor Barrie and Holmes, Dr William and Muir, Professor Keith and McCabe, Dr Chris and Santosh, Dr Celestine
Authors: Robertson, C. A., McCabe, C., Gallagher, L., Lopez-Gonzalez, M. d., Holmes, W. M., Condon, B., Muir, K. W., Santosh, C., and Macrae, I. M.
Subjects:R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
College/School:College of Medical Veterinary and Life Sciences > Institute of Neuroscience and Psychology
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Clinical Specialities
Journal Name:Journal of Cerebral Blood Flow and Metabolism
Publisher:Nature
ISSN:0271-678X
Published Online:11 May 2011
Copyright Holders:Copyright © 2011 Nature
First Published:First published in Journal of Cerebral Blood Flow and Metabolism 31:1778-1787
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
455001Imaging the ischaemic penumbra using BOLD MRI with oxygen challenge as a biotracerI MacraeMedical Research Council (MRC)G0700439Institute of Neuroscience and Psychology