Azole resistance of aspergillus fumigatus biofilms is partly associated with efflux pump activity

Rajendran, R., Mowat, E., McCulloch, E., Lappin, D.F., Jones, B., Lang, S., Majithiya, J.B., Warn, P., Williams, C. and Ramage, G. (2011) Azole resistance of aspergillus fumigatus biofilms is partly associated with efflux pump activity. Antimicrobial Agents and Chemotherapy, 55(5), pp. 2092-2097. (doi:10.1128/AAC.01189-10)

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Abstract

This study investigated the phase-dependent expression and activity of efflux pumps in Aspergillus fumigatus treated with voriconazole. Fourteen strains were shown to become increasingly resistant in the 12-h (16- to 128-fold) and 24-h (> 512-fold) phases compared to 8-h germlings. An Ala-Nap uptake assay demonstrated a significant increase in efflux pump activity in the 12-h and 24-h phases (P < 0.0001). The efflux pump activity of the 8-h germling cells was also significantly induced by voriconazole (P < 0.001) after 24 h of treatment. Inhibition of efflux pump activity with the competitive substrate MC-207,110 reduced the voriconazole MIC values for the A. fumigatus germling cells by 2- to 8-fold. Quantitative expression analysis of AfuMDR4 mRNA transcripts showed a phase-dependent increase as the mycelial complexity increased, which was coincidental with a strain-dependent increase in azole resistance. Voriconazole also significantly induced this in a time-dependent manner (P < 0.001). Finally, an in vivo mouse biofilm model was used to evaluate efflux pump expression, and it was shown that AfuMDR4 was constitutively expressed and significantly induced by treatment with voriconazole after 24 h (P < 0.01). Our results demonstrate that efflux pumps are expressed in complex A. fumigatus biofilm populations and that this contributes to azole resistance. Moreover, voriconazole treatment induces efflux pump expression. Collectively, these data may provide evidence for azole treatment failures in clinical cases of aspergillosis.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Lappin, Dr David and Ramage, Professor Gordon and Rajendran, Dr Ranjith
Authors: Rajendran, R., Mowat, E., McCulloch, E., Lappin, D.F., Jones, B., Lang, S., Majithiya, J.B., Warn, P., Williams, C., and Ramage, G.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:Antimicrobial Agents and Chemotherapy
Journal Abbr.:Antimicrob Agents Chemother
Publisher:American Society for Microbiology
ISSN:0066-4804
ISSN (Online):1098-6596
Published Online:14 February 2011

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