Rochat, R.H., Liu, X., Murata, K., Nagayama, K., Rixon, F.J. and Chiu, W. (2011) Seeing the portal in Herpes Simplex Virus Type 1 B capsids. Journal of Virology, 85(4), pp. 1871-1874. (doi: 10.1128/JVI.01663-10) (PMID:21106752) (PMCID:PMC3028901)
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Abstract
Resolving the non-icosahedral components in large icosahedral viruses remains a technical challenge in structural virology. We have used the emerging technique of Zernike phase contrast electron cryo-microscopy to enhance image contrast of ice-embedded HSV-1 capsids. Image reconstruction enabled us to retrieve the structure of the unique portal vertex in the context of the icosahedral capsid, and for the first time show the subunit organization of a portal in a virus infecting eukaryotes. Our map unequivocally resolves the twelve-subunit portal situated beneath one of the pentameric vertices, thus removing uncertainty over the location and stoichiometry of the herpesvirus portal.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Rixon, Dr Frazer |
| Authors: | Rochat, R.H., Liu, X., Murata, K., Nagayama, K., Rixon, F.J., and Chiu, W. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Life Sciences |
| Journal Name: | Journal of Virology |
| Publisher: | American Society for Microbiology |
| ISSN: | 0022-538X |
| ISSN (Online): | 1098-5514 |
| Published Online: | 24 November 2010 |
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