A performance evaluation of commercial fibrinogen reference preparations and assays for Clauss and PT-derived fibrinogen

Mackie, I.J., Lawrie, A.S., Kitchen, S., Gaffney, P.J., Howarth, D., Lowe, G.D.O., Martin, J., Purdy, G., Rigsby, P. and Rumley, A. (2002) A performance evaluation of commercial fibrinogen reference preparations and assays for Clauss and PT-derived fibrinogen. Thrombosis and Haemostasis, 87(6), pp. 997-1005.

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Publisher's URL: https://th.schattauer.de/contents/archive/issue/806/issue/special/manuscript/3149/show.html

Abstract

The wide availability of fibrinogen estimations based on the prothrombin time (PT-Fg) has caused concern about the variability and clinical utility of fibrinogen assays. In a multi-centre study, we investigated fibrinogen assays using various reagents and analysers, Clauss assays generally gave good agreement, although one reagent gave 15-30% higher values in DIC and thrombolysis. Two commercial reference preparations had much lower potencies than the manufacturers declared, and plasma turbidity influenced parallelism in some Clauss assays, PT-Fg assays gave higher values than Clauss and showed calibrant dependent effects, the degree of disparity correlating with calibrant and test sample turbidity. Analyser and thromboplastin dependent differences were noted. The relationship between Clauss and PT-Fg assays was sigmoid, and the plateau of maximal PT-Fg differed by about 2 g/l between reagents. ELISA and immunonephelometric assays correlated well, but with a high degree of scatter. Antigen levels were higher than Clauss, but slightly lower than PT-Fg assays, which appeared to be influenced by degraded fibrinogen. Clauss assays are generally reproducible between centres, analysers and reagents, but PT-Fg assays are not reliable in clinical settings.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Rumley, Dr Ann and Lowe, Professor Gordon
Authors: Mackie, I.J., Lawrie, A.S., Kitchen, S., Gaffney, P.J., Howarth, D., Lowe, G.D.O., Martin, J., Purdy, G., Rigsby, P., and Rumley, A.
Subjects:R Medicine > RC Internal medicine
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
Journal Name:Thrombosis and Haemostasis
Publisher:Schattauer GmbH
ISSN:0340-6245
Copyright Holders:Copyright © 2002 Schattauer GmbH
First Published:First published in Thrombosis and Haemostasis 87(6):997-1005
Publisher Policy:Reproduced with the permission of the Publisher.

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