Biomimetic microtopography to enhance osteogenesis in vitro

Wilkinson, A., Hewitt, R. N., McNamara, L. E., McCloy, D., Meek, R.M. D. and Dalby, M. J. (2011) Biomimetic microtopography to enhance osteogenesis in vitro. Acta Biomaterialia, 7(7), pp. 2919-2925. (doi:10.1016/j.actbio.2011.03.026)

Full text not currently available from Enlighten.

Publisher's URL: http://dx.doi.org/10.1016/j.actbio.2011.03.026

Abstract

Biomimicry is being used in the next generation of biomaterials. Tuning material surface features such as chemistry, stiffness and topography allow the control of cell adhesion, proliferation, growth and differentiation. Here, microtopographical features with nanoscale depths, similar in scale to osteoclast resorption pits, were used to promote in vitro bone formation in basal medium. Primary human osteoblasts were used to represent an orthopaedically relevant cell type and analysis of adhesions, cytoskeleton, osteospecific proteins (phospho-Runx2 and osteopontin) and mineralisation (alizarin red) was performed. The results further demonstrate the potential for biomimicry in material design and show that the osteoblast response can be tuned from changes in feature size.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McNamara, Dr Laura and Wilkinson, Dr Andrew and Meek, Professor Dominic and Dalby, Professor Matthew
Authors: Wilkinson, A., Hewitt, R. N., McNamara, L. E., McCloy, D., Meek, R.M. D., and Dalby, M. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Acta Biomaterialia
Publisher:Elsevier
ISSN:1742-7061
Published Online:01 April 2011

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
502891Micro- and nano-patterning of titanium surfaces for optimal osseointegration of orthopaedic implantsMatthew DalbyEngineering & Physical Sciences Research Council (EPSRC)EP/G048703/1RI MOLECULAR CELL & SYSTEMS BIOLOGY