Separation of parasites from human blood using deterministic lateral displacement

Holm, S. H., Beech, J. P., Barrett, M. P. and Tegenfeldt, J. O. (2011) Separation of parasites from human blood using deterministic lateral displacement. Lab on a Chip, 11(7), pp. 1326-1332. (doi:10.1039/c0lc00560f)

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Publisher's URL: http://dx.doi.org/10.1039/c0lc00560f

Abstract

We present the use of a simple microfluidic technique to separate living parasites from human blood. Parasitic trypanosomatids cause a range of human and animal diseases. African trypanosomes, responsible for human African trypanosomiasis (sleeping sickness), live free in the blood and other tissue fluids. Diagnosis relies on detection and due to their often low numbers against an overwhelming background of predominantly red blood cells it is crucial to separate the parasites from the blood. By modifying the method of deterministic lateral displacement, confining parasites and red blood cells in channels of optimized depth which accentuates morphological differences, we were able to achieve separation thus offering a potential route to diagnostics.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Barrett, Professor Michael
Authors: Holm, S. H., Beech, J. P., Barrett, M. P., and Tegenfeldt, J. O.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Lab on a Chip
Publisher:Royal Society of Chemistry
ISSN:1473-0197
ISSN (Online):1473-0189
Published Online:18 February 2011

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