In vivo real-time multiphoton imaging of t lymphocytes in the mouse brain after experimental stroke

Fumagalli, S. et al. (2011) In vivo real-time multiphoton imaging of t lymphocytes in the mouse brain after experimental stroke. Stroke, 42(5), pp. 1429-1436. (doi: 10.1161/STROKEAHA.110.603704)

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Background and Purpose-To gain a better understanding of T cell behavior after stroke, we have developed real-time in vivo brain imaging of T cells by multiphoton microscopy after middle cerebral artery occlusion. Methods-Adult male hCD2-GFP transgenic mice that exhibit green fluorescent protein-labeled T cells underwent permanent left distal middle cerebral artery occlusion by electrocoagulation (n = 6) or sham surgery (n = 6) and then multiphoton laser imaging 72 hours later.<p></p> Results-Extravasated T cell number significantly increased after middle cerebral artery occlusion versus sham. Two T cell populations existed after middle cerebral artery occlusion, possibly driven by 2 T cell subpopulations; 1 had significantly lower and the other significantly higher track velocity and displacement rate than sham.<p></p> Conclusions-The different motilities and behaviors of T cells observed using our imaging approach after stroke could reveal important mechanisms of immune surveillance for future therapeutic exploitations.<p></p>

Item Type:Articles
Glasgow Author(s) Enlighten ID:Garside, Professor Paul and Brewer, Professor James and Maffia, Professor Pasquale and Coles, Dr Jonathan and Dever, Dr Gary
Authors: Fumagalli, S., Coles, J. A., Ejlerskov, P., Ortolano, F., Bushell, T. J., Brewer, J. M., De Simoni, M.-G., Dever, G., Garside, P., Maffia, P., and Carswell, H. V.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Stroke
Publisher:American Heart Association
ISSN (Online):1524-4628
Published Online:24 March 2011

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