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Characterization and genomic mapping of genes and pseudogenes of a new human protein tyrosine phosphatase

Zhao, Z., Lee, C.C., Monckton, D.G., Yazdani, A., Coolbaugh, M., Li, X., Bailey, J., Shen, Y., and Caskey, C.T. (1996) Characterization and genomic mapping of genes and pseudogenes of a new human protein tyrosine phosphatase. Genomics, 35 (1). 172 -181. ISSN 0888-7543 (doi:10.1006/geno.1996.0336)

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Publisher's URL: http://dx.doi.org/10.1006/geno.1996.0336

Abstract

Previously described protein tyrosine phosphatases (PTPs) are classified into three types according to their sequence homology and structural features. Here we describe the characterization of genes and pseudogenes of a member of a fourth type of PTP, designated protein tyrosine phosphatase 4A (PTP4A). The 167-amino-acid human PTP4A bears the signature active site of all PTPs, but does not show any other sequence homology to any of the previously described PTPs. Two cDNAs encoding PTP4Athat differed in their noncoding regions were isolated. Another cDNA that has a high level of sequence identity with these two cDNAs and a deletion in the coding region was also isolated. Northern analysis using a probe from a common 3'-untranslated region of the cDNAs recognized mRNAs of about 2 and 4 kb. Both species of mRNA were seen in all human adult and fetal tissues tested. Fluorescence in situ hybridization mapping of the corresponding yeast artificial chromosome clones and sequence-tagged site analysis suggested that one of the PTP4A coding genes is located at 1p35 and the other is on chromosome 11. A processed pseudogene for PTP4A was found in the BRCA1 region of 17q21 and shares 96% sequence identity to one of the PTP4A coding cDNAs. Our studies also suggest the existence of another processed pseudogene on chromosome 11.

Item Type:Article
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Li, Dr Xu and Monckton, Prof Darren
Authors: Zhao, Z., Lee, C.C., Monckton, D.G., Yazdani, A., Coolbaugh, M., Li, X., Bailey, J., Shen, Y., and Caskey, C.T.
Subjects:Q Science > QH Natural history > QH426 Genetics
Q Science > QP Physiology
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Genomics
ISSN:0888-7543

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