A structural analysis of M protein in coronavirus assembly and morphology

Neuman, B. W. et al. (2011) A structural analysis of M protein in coronavirus assembly and morphology. Journal of Structural Biology, 174(1), pp. 11-22. (doi:10.1016/j.jsb.2010.11.021) (PMID:21130884)

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Abstract

The M protein of coronavirus plays a central role in virus assembly, turning cellular membranes into workshops where virus and host factors come together to make new virus particles. We investigated how M structure and organization is related to virus shape and size using cryo-electron microscopy, tomography and statistical analysis. We present evidence that suggests M can adopt two conformations and that membrane curvature is regulated by one M conformer. Elongated M protein is associated with rigidity, clusters of spikes and a relatively narrow range of membrane curvature. In contrast, compact M protein is associated with flexibility and low spike density. Analysis of several types of virus-like particles and virions revealed that S protein, N protein and genomic RNA each help to regulate virion size and variation, presumably through interactions with M. These findings provide insight into how M protein functions to promote virus assembly. (C) 2010 Elsevier Inc. All rights reserved

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Bhella, Dr David
Authors: Neuman, B. W., Kiss, G., Kunding, A. H., Bhella, D., Baksh, M. F., Connelly, S., Droese, B., Klaus, J. P., Makino, S., Sawicki, S. G., Siddell, S. G., Stamou, D. G., Wilson, I. A., Kuhn, P., and Buchmeier, M. J.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Journal of Structural Biology
Publisher:Elsevier Inc.
ISSN:1047-8477
ISSN (Online):1095-8657
Published Online:03 December 2010

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
656541Structural studies of human viruses and host interactionsDavid BhellaMedical Research Council (MRC)MC_UU_12014/7MVLS III - CENTRE FOR VIRUS RESEARCH