Matrix-free mass spectrometric imaging using laser desorption ionisation Fourier transform ion cyclotron resonance mass spectrometry

Goodwin, R.J.A., Pitt, A.R., Harrison, D., Weidt, S.K., Langridge-Smith, P.R.R., Barrett, M.P. and Mackay, C.L. (2011) Matrix-free mass spectrometric imaging using laser desorption ionisation Fourier transform ion cyclotron resonance mass spectrometry. Rapid Communications in Mass Spectrometry, 25(7), pp. 969-972. (doi:10.1002/rcm.4939)

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Publisher's URL: http://dx.doi.org/10.1002/rcm.4939

Abstract

Mass spectrometry imaging (MSI) is a powerful tool in metabolomics and proteomics for the spatial localization and identification of pharmaceuticals, metabolites, lipids, peptides and proteins in biological tissues. However, sample preparation remains a crucial variable in obtaining the most accurate distributions. Common washing steps used to remove salts, and solvent-based matrix application, allow analyte spreading to occur. Solvent-free matrix applications can reduce this risk, but increase the possibility of ionisation bias due to matrix adhesion to tissue sections. We report here the use of matrix-free MSI using laser desorption ionisation performed on a 12 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. We used unprocessed tissue with no post-processing following thaw-mounting on matrix-assisted laser desorption ionisation (MALDI) indium-tin oxide (ITO) target plates. The identification and distribution of a range of phospholipids in mouse brain and kidney sections are presented and compared with previously published MALDI time-of-flight (TOF) MSI distributions.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Pitt, Dr Andrew and Goodwin, Dr Richard and Barrett, Professor Michael
Authors: Goodwin, R.J.A., Pitt, A.R., Harrison, D., Weidt, S.K., Langridge-Smith, P.R.R., Barrett, M.P., and Mackay, C.L.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Rapid Communications in Mass Spectrometry
ISSN:0951-4198

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