CE-MS in biomarker discovery, validation, and clinical application

Mischak, H. and Schanstra, J.P. (2010) CE-MS in biomarker discovery, validation, and clinical application. Proteomics Clinical Applications, 5(1-2), pp. 9-23. (doi: 10.1002/prca.201000058)

Abstract

CE coupled MS (CE-MS) has become an increasingly employed technology in proteome analysis with focus on the identification of biomarker peptides in clinical proteomics. In this review, we will cover technical aspects of CE-MS coupling and highlight the improvements made in the last few years. We examine CE-MS from an application point of view, and evaluate its merits and vices for biomarker discovery and clinical applications. We discus the principal theoretical and practical obstacles encountered when employing CE-MS (and most other proteomic technologies) for the analysis of body fluids for biomarker discovery. We will present several examples of a successful application of CE-MS for biomarker discovery, implications for disease diagnosis, prognosis, and therapy evaluation, and will discuss current challenges and possible future improvements

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Mischak, Professor Harald
• Authors: Mischak, H., and Schanstra, J.P.
• College/School: College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
• Journal Name: Proteomics Clinical Applications
• ISSN: 1862-8346