Magnetic resonance imaging to assess blood-brain barrier damage in murine trypanosomiasis

Rodgers, J. , McCabe, C. , Gettinby, G., Bradley, B., Condon, B. and Kennedy, P. G.E. (2011) Magnetic resonance imaging to assess blood-brain barrier damage in murine trypanosomiasis. American Journal of Tropical Medicine and Hygiene, 84(2), pp. 344-350. (doi:10.4269/ajtmh.2011.10-0487)

Rodgers, J. , McCabe, C. , Gettinby, G., Bradley, B., Condon, B. and Kennedy, P. G.E. (2011) Magnetic resonance imaging to assess blood-brain barrier damage in murine trypanosomiasis. American Journal of Tropical Medicine and Hygiene, 84(2), pp. 344-350. (doi:10.4269/ajtmh.2011.10-0487)

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Publisher's URL: http://dx.doi.org/10.4269/ajtmh.2011.10-0487

Abstract

The ability of trypanosomes to invade the brain and induce an inflammatory reaction is well-recognized. This study uses magnetic resonance imaging (MRI) in conjunction with a murine model of central nervous system (CNS) stage trypanosomiasis to investigate this phenomenon at the level of the blood-brain barrier (BBB). Mice were scanned before and after administration of the contrast agent. Signal enhancement maps were generated, and the percentage signal change was calculated. The severity of the neuroinflammation was also assessed. Statistical analysis of the signal change data revealed a significantly (P = 0.028) higher signal enhancement in mice at 28 days post-infection (least squares mean = 26.709) compared with uninfected animals (6.298), indicating the presence of BBB impairment. Leukocytes were found in the meninges and perivascular space of some blood vessels in the infected mice. This study shows that the integrity of the BBB is compromised during CNS stage trypanosomiasis and that the impairment does not correlate with inflammatory cell infiltration.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Condon, Professor Barrie and Kennedy, Professor Peter and Bradley, Mrs Barbara and McCabe, Dr Chris and Rodgers, Dr Jean
Authors: Rodgers, J., McCabe, C., Gettinby, G., Bradley, B., Condon, B., and Kennedy, P. G.E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Neuroscience and Psychology
Journal Name:American Journal of Tropical Medicine and Hygiene
Publisher:American Society of Tropical Medicine and Hygiene
ISSN:0002-9637
ISSN (Online):1476-1645
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
443321Combination chemotherapy of human African trypanosomiasis - the potential of drug synergism and the blood-brain barrierPeter KennedyMedical Research Council (MRC)G0601059Institute of Infection Immunity and Inflammation