Galectin-3 is a negative regulator of lipopolysaccharide-mediated inflammation

Li, Y., Komai-Koma, M., Gilchrist, D.S., Hsu, D.K., Liu, F.-T., Spingall, T. and Xu, D. (2008) Galectin-3 is a negative regulator of lipopolysaccharide-mediated inflammation. Journal of Immunology, 181(4), pp. 2781-2789.

Full text not currently available from Enlighten.

Publisher's URL: http://www.jimmunol.org/content/181/4/2781.full

Abstract

Galectin-3 is a beta-galactoside-binding lectin that plays an important role in inflammatory diseases. It also interacts with the surface carbohydrates of many pathogens, including LPS. However, its role in infection is not fully understood. Data presented herein demonstrate for the first time that galectin-3 is a negative regulator of LPS-induced inflammation. Galectin-3 is constitutively produced by macrophages and directly binds to LPS. Galectin-3-deficient macrophages had markedly elevated LPS-induced signaling and inflammatory cytokine production compared with wild-type cells, which was specifically inhibited by the addition of recombinant galectin-3 protein. In contrast, blocking galectin-3 binding sites by using a neutralizing Ab or its ligand, beta-lactose, enhanced LPS-induced inflammatory cytokine expression by wild-type macrophages. In vivo, mice lacking galectin-3 were more susceptible to LPS shock associated with excessive induction of inflammatory cytokines and NO production. However, these changes conferred greater resistance to Salmonella infection. Thus, galectin-3 is a previously unrecognized, naturally occurring, negative regulator of LPS function, which protects the host from endotoxin shock but, conversely, favors Salmonella survival.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Xu, Dr Damo and Gilchrist, Dr Derek and Komai-Koma, Dr Mousa
Authors: Li, Y., Komai-Koma, M., Gilchrist, D.S., Hsu, D.K., Liu, F.-T., Spingall, T., and Xu, D.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Immunology
ISSN:0022-1767
ISSN (Online):1550-6606

University Staff: Request a correction | Enlighten Editors: Update this record