A fast brassinolide-regulated response pathway in the plasma membrane of Arabidopsis thaliana

Caesar, K., Elgass, K., Chen, Z., Huppenberger, P., Witthöft, J., Schleifenbaum, F., Blatt, M.R. , Oecking, C. and Harter, K. (2011) A fast brassinolide-regulated response pathway in the plasma membrane of Arabidopsis thaliana. Plant Journal, 66(3), pp. 528-540. (doi:10.1111/j.1365-313X.2011.04510.x)

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Publisher's URL: http://dx.doi.org/10.1111/j.1365-313X.2011.04510.x

Abstract

Summary To understand molecular processes in living plant cells, quantitative spectro-microscopic technologies are required. By combining fluorescence lifetime spectroscopy with confocal microscopy, we studied the subcellular properties and function of a GFP-tagged variant of the plasma membrane-bound brassinosteroid receptor BRI1 (BRI1-GFP) in living cells of Arabidopsis seedlings. Shortly after adding brassinolide we observed a BRI1-dependent cell wall expansion, which precedes cell elongation. In parallel, the fluorescence lifetime of BRI1-GFP decreased, indicating an alteration in the receptorGǦs physico-chemical environment. The parameter modulating the fluorescence lifetime of BRI1-GFP was found to be a BL-induced hyperpolarisation of the plasma membrane. Furthermore, for the induction of hyperpolarisation and cell wall expansion, the activation of the plasma membrane P-ATPase was necessary. This activation required BRI1 kinase activity and was mediated by BL-modulated interaction of BRI1 with the P-ATPase. Our results can be combined in a model, which suggests that there is a fast BL-regulated signal response pathway within the plasma membrane that links BRI1 with the P-ATPase for the regulation of cell wall expansion

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Blatt, Professor Michael and Chen, Dr Zhonghua
Authors: Caesar, K., Elgass, K., Chen, Z., Huppenberger, P., Witthöft, J., Schleifenbaum, F., Blatt, M.R., Oecking, C., and Harter, K.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Plant Journal
ISSN:0960-7412

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
453651Systems analysis of guard cell oscillatory mechanics in stomatal dynamicsMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/F001673/1Institute of Molecular Cell and Systems Biology
452562Analysis of membrane traffic in adaptive stress tolerance in plantsMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/F001630/1Institute of Molecular Cell and Systems Biology