Technical, bioinformatical and statistical aspects of liquid chromatography–mass spectrometry (LC–MS) and capillary electrophoresis-mass spectrometry (CE-MS) based clinical proteomics: A critical assessment

Dakna, M., He, Z., Yu, W.C., Mischak, H. and Kolch, W. (2009) Technical, bioinformatical and statistical aspects of liquid chromatography–mass spectrometry (LC–MS) and capillary electrophoresis-mass spectrometry (CE-MS) based clinical proteomics: A critical assessment. Journal of Chromatography B, 877(13), pp. 1250-1258. (doi: 10.1016/j.jchromb.2008.10.048)

Abstract

The search for biomarkers in biological fluids that can be used for disease diagnosis and prognosis using mass spectrometry has emerged to become a state-of-the-art methodology for clinical proteomics. Poor cross platform comparison of the findings, however, makes the need for comparison studies probably as urgent as the need for new ones. It is now increasingly recognized that standardized statistical and bioinformatics approaches during data processing are of utmost importance for such comparisons. This paper reviews two of the currently most promising methods, namely LC-MS and CE-MS techniques, and software tools used to analyze the huge amount of data they generate. We further review the statistical issues of feature selection and sample classification

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Kolch, Prof Walter and Mischak, Professor Harald
• Authors: Dakna, M., He, Z., Yu, W.C., Mischak, H., and Kolch, W.
• College/School: College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health; College of Medical Veterinary and Life Sciences
• Journal Name: Journal of Chromatography B
• ISSN: 1570-0232