Experimental vaccination of sheep and cattle against tick infestation using recombinant 5′-nucleotidase

Hope, M., Jiang, X., Gough, J., Cadogan, L., Josh, P., Jonsson, N. and Willadsen, P. (2010) Experimental vaccination of sheep and cattle against tick infestation using recombinant 5′-nucleotidase. Parasite Immunology, 32(2), pp. 135-142. (doi:10.1111/j.1365-3024.2009.01168.x)

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Abstract

Limited prior evidence suggests that 5′-nucleotidase, an ectoenzyme principally located in the Malpighian tubules of the tick Rhipicephalus (Boophilus) microplus, could be an effective antigen in an anti-tick vaccine. To assess this, recombinant 5′-nucleotidase was expressed in Escherichia coli and used in vaccination trials with both sheep and cattle. Vaccinated sheep were challenged with freshly moulted adult ticks. Those with high titres of anti-nucleotidase antibodies showed significant protection against tick infestation, although protection was less than that found with the previously characterized antigen, Bm86. Cattle were vaccinated, in separate groups, with 5′-nucleotidase, Bm86 and both antigens combined. Cattle, as the natural host, were challenged with larval ticks. Although Bm86 showed typical efficacy, no significant protection was seen in cattle vaccinated with 5′-nucleotidase. Cattle receiving a dual antigen formulation were no better protected than those receiving Bm86 alone. One possible reason for the difference between host species, namely antibody titre, was examined and shown to be an unlikely explanation. This demonstrates a limitation of using a model host like sheep in vaccine studies.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Jonsson, Professor Nicholas
Authors: Hope, M., Jiang, X., Gough, J., Cadogan, L., Josh, P., Jonsson, N., and Willadsen, P.
Subjects:Q Science > QR Microbiology > QR180 Immunology
S Agriculture > SF Animal culture > SF600 Veterinary Medicine
College/School:College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Parasite Immunology
Publisher:Wiley-Blackwell Publishing Ltd.
ISSN:0141-9838
ISSN (Online):1365-3024
Published Online:11 January 2010
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