Molecular interactions of SHP1 and SHP2 in IL-3-signalling

Wheadon, H. , Paling, N.R.D. and Welham, M.J. (2002) Molecular interactions of SHP1 and SHP2 in IL-3-signalling. Cellular Signalling, 14(3), pp. 219-229. (doi:10.1016/S0898-6568(01)00241-8)

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Abstract

SHP1 and SHP2 tyrosine phosphatases have both been implicated in signalling pathways downstream of the interleukin-3 (IL-3) receptor. We have investigated the co-association of SHP1 and SHP2 with tyrosine-phosphorylated proteins in IL-3-dependent BaF/3 cells. We demonstrate that both SHP1 and SHP2 associate with Aic2A (β chain of the IL-3 receptor), Gab2 and the paired inhibitory receptor B (PIR-B). The individual SH2 domains of SHP2 can independently bind Gab2, potentially important for the adapter function of SHP2. Association of both phosphatases with Aic2A and Gab2 increases upon IL-3 treatment. Recruitment of SHP1 to PIR-B also increases in response to IL-3, suggesting a functional link between inhibitory and cytokine receptor signalling. Aic2A is a rapid target for dephosphorylation following IL-3 stimulation and substrate-trapping versions of both phosphatases identify Aic2A and Gab2 as substrates for SHP1 and SHP2. These studies suggest that SH2–domain interactions are important for targetting these phosphatases to their substrates.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Wheadon, Dr Helen
Authors: Wheadon, H., Paling, N.R.D., and Welham, M.J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Cellular Signalling
ISSN:0898-6568
Published Online:21 January 2002

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