Howard, A., Townes, C., Milona, P., Nile, C.J. , Michailidis, G. and Hall, J. (2010) Expression and functional analyses of liver expressed antimicrobial peptide-2 (LEAP-2) variant forms in human tissues. Cellular Immunology, 261(2), pp. 128-133. (doi: 10.1016/j.cellimm.2009.11.010)
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Abstract
The antimicrobial peptide Liver Expressed Antimicrobial Peptide-2 (LEAP-2) is proposed to function as part of the vertebrate innate immune system. However, the highly conserved nature of the LEAP-2 peptide primary structure among vertebrates suggests more fundamental physiological roles. RT-PCR analyses confirmed expression of LEAP-2 mRNA variants in human gastro-intestinal (GI) epithelial tissues and THP-1 monocytes. Three cDNA products indicative of at least three different spliced transcripts were observed. Translation of the cDNA sequences supported synthesis of transcripts encoding the secreted LEAP-2 peptide and two variants lacking signal sequences suggesting intracellular localisation. The synthesis and cytoplasmic localisation of LEAP-2 peptides in epithelia was supported by immunohistochemical analyses. Functional data suggested that LEAP-2 is not involved in the physiological response of GI epithelia to iron, nor is it mitogenic for epithelial cells or chemotactic for THP-1 monocytes. However, changes in the LEAP-2 transcript patterns associated with the challenge of THP-1 monocytes with lipopolysaccharide (100 ng/ml) were supportive of the peptides having multiple roles in the innate immune response.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Nile, Dr Christopher |
| Authors: | Howard, A., Townes, C., Milona, P., Nile, C.J., Michailidis, G., and Hall, J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School |
| Journal Name: | Cellular Immunology |
| ISSN: | 0008-8749 |
| ISSN (Online): | 1090-2163 |
| Published Online: | 03 December 2009 |
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